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Cell No. : Cell Name
GMC0007 : WS3RGB  update : 2018/07/06
CommentAccelerated aging in vitro, Werner's syndrome.
Comment from the depositor
Terms and conditionsRequired written permission from the approver.
Remarks
approver's address
×
English
Address
Toin University Of Yokohama

1614 Kurogane-machi, Aoba-ku, Yokohama, Kanagawa, 225-8502 Japan
Dr.Goto, Makoto
Fax. +81-45-974-5193
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Goto, Makoto
Originator Ohno, Tadao
Year of deposit 1987
Another name RCB0155
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 42 years
Tissue skin
Genetics Werner's syndrome
Classification normal
Year of origin 1987
History 1/23/87 biopsy by M. Goto at N
Lifespan finite
Crisis PDL 33.5 PDL
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Reference information Reference 1
User's Publication 1


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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User's Publication
2825  Zhang Z, Hirano K, Tsukamoto K, Ikegami C, Koseki M, Saijo K, Ohno T, Sakai N, Hiraoka H, Shimomura I, Yamashita S  Defective cholesterol efflux in Werner syndrome fibroblasts and its phenotypic correction by Cdc42, a RhoGTPase.  Exp Gerontol  2005  40:286-94  PubMed ID: 15820609



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