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Cell No. : Cell Name
GMC0007 : WS3RGB  update : 2024/08/27
CommentAccelerated aging in vitro, Werner's syndrome.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
1-11-11-101 Kitazakae, Urayasy-shi, Chiba, 279-0002 Japan
Urayasu Rheumatology Clinic
E-mail. werner.goto@gmail.com
Dr.GOTO Makoto
Order Form Order Form(C-0052.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Goto, Makoto
Originator Ohno, Tadao
Year of deposit 1987
Another name RCB0155
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 42 years
Tissue skin
Genetics Werner's syndrome
Classification normal
Year of origin 1987
History 1/23/87 biopsy by M. Goto at N
Lifespan finite
Crisis PDL 33.5 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T330
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
STR(human) OK
Reference information Reference 4
User's Publication 1


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014   DOI: 10.5582/irdr.2018.01092
919  Makoto Goto, Junji Chiba, Masaaki Matsuura, Sachiko Iwaki-Egawa, Yasuhiro Watanabe  Inflammageing assessed by MMP9 in normal Japanese individuals and the patients with Werner syndrome  Intractable Rare Dis Res  2016  5(2):103-8  PubMed ID: 27195193   DOI: 10.5582/irdr.2016.01028
918  M Goto, Y Ishikawa, M Sugimoto, Y Furuichi  Werner syndrome: a changing pattern of clinical manifestations in Japan (1917~2008)  Biosci Trends  2013  7(1):13-22  PubMed ID: 23524889  
920  M Goto, S Iwaki-Egawa, Y Watanabe  Ageing in Werner syndrome  Biosci Trends  2012  6(1):33-7  PubMed ID: 22426101   DOI: 10.5582/bst.2012.v6.1.33

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User's Publication
2825  Zhang Z, Hirano K, Tsukamoto K, Ikegami C, Koseki M, Saijo K, Ohno T, Sakai N, Hiraoka H, Shimomura I, Yamashita S  Defective cholesterol efflux in Werner syndrome fibroblasts and its phenotypic correction by Cdc42, a RhoGTPase.  Exp Gerontol  2005  40:286-94  PubMed ID: 15820609   DOI: 10.1016/j.exger.2005.01.011


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