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Cell No. : Cell Name
GMC0002 : A0082  update : 2021/05/20
CommentHuman primary adherent cells derived from Werner syndrome patient's skin. The same patient as GMC0001.
Comment from the depositor
Terms and conditionsRequired written permission from the approver .
Remarks
approver's address
×
English
Address
1-11-11-101 Kitazakae, Urayasy-shi, Chiba, 279-0002 Japan
Urayasu Rheumatology Clinic
E-mail. werner.goto@gmail.com
Dr.Goto, Makoto
Order Form Order Form(C-0052.pdf)   Approval Form(C-0006.pdf)   MTA(C-0053.pdf)   MTA(C-0053p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Goto, Makoto
Originator Saijo, Kaoru
Year of deposit 2008
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 70 years
Tissue skin
Case history Cataract,Characteristic skin,Mellitus
Genetics Werner's syndrome
Classification normal
Year of origin 2007
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T335
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4062  Goto M, Chiba J, Matsuura M, Iwaki-Egawa S, Watanabe Y.  Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome.  Intractable Rare Dis Res  2018  7:229-235  PubMed ID: 30560014

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