Back
|
Cell No. : Cell Name
APS0008 : iPS-L1 update : 2025/01/15 |
||||
| Comment | Rabbit induced pluripotent stem (iPS) cell line. Application consideration. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1)The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 2010;285:31362-31369) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 5) When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002). | |||
| Remarks | ||||
| approver's address |
×
|
|||
| Order Form | Order Form(C-0033.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Honda, Arata | ||
| Originator | Honda, Arata | |||
| Year of deposit | 2011 | |||
| Animal | _rabbit < Mammals | |||
| Genus | Oryctolagus | |||
| Species | cuniclus | |||
| Gender | Unknown | |||
| Tissue | liver | |||
| Classification | iPS | |||
| Recombinant | recombinant | |||
| Exogene | pCSII-EF-MCS-IRES2-Venus lentiviral vector, human-Oct3/4, -Sox2, -Klf4, -c-Myc | Lifespan | infinite | |
| Morphology | ES-like | |||
| Cellosaurus(Expasy) | CVCL_B969 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 8ng/ml human basic FGF | |||
| Antibiotics | Free | Passage method | Use DMEM/HamF12 (not PBS(-)) for washing. 0.1% Trypsin (DMEM/HamF12) | |
| Coating dish | 0.1% gelatin coated dish | Culture information | Passage cell No | 0.5-1 x 10 4 cells/60 mm dish |
| SC frequency | Subculture : once/3-4 days, Medium Renewal : every day | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 6 % | |||
| Feeder cells | MEF | |||
| Feeder treatment | X-rays 5000R (or MMC) | |||
| Feeder seed cells | 1-2 x 10 5 cells/60 mm dish | |||
| Freeze medium | Medium : 2 x FM1 = 1 : 1 | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Isozyme | LD, NP | |||
| Chromosome mode | 45(49), 46(1) ,46,X,-Xor-Y,add(2)(p),add(4)(p),+17,add(17)(p),i(20)(q),+mar1,+mar2[1] etc. | |||
| Images | ||||
|
|
|
|||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
Top
Top
Back
CELL BANK Website