Cell No. : Cell Name
APS0007 : iPS-Hep-FB/Ng/gfp-103C-1
update : 2023/03/28
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| Comment | Mouse iPS cell line derived from liver cells. Expressing GFP by CAG promoter. βgeo is knocked-in at Fbx15 locus. GFP and puromycin resistant gene can be expressed by Nanog promoter.Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0033.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Originator |
Yamanaka, Shinya
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| Year of deposit |
2009
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| Animal |
_mouse
< Mammals
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| Gender |
Male
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| Age at sampling |
21 weeks
|
| Tissue |
liver
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
pMXs, Oct3/4, Sox2, Klf4, c-Myc, GFP, βgeo gene
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_T784
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 6 cells/100 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
|
OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
5
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| User's Publication |
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11029
Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells
Journal of Hard Tissue Biology
2018
27, 1-10
DOI: 10.2485/jhtb.27.1
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11163
Zhou Q, Ye X, Ran Q, Kitahara A, Matsumoto Y, Moriyama M, Ajioka Y, Saijo Y.
Trachea Engineering Using a Centrifugation Method and Mouse-Induced Pluripotent Stem Cells.
Tissue Eng Part C Methods
2018
24(9):524-533
PubMed ID: 30101671
DOI: 10.1089/ten.TEC.2018.0115
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7075
Takagi R, Ishimaru J, Sugawara A, Toyoshima KE, Ishida K, Ogawa M, Sakakibara K, Asakawa K, Kashiwakura A, Oshima M, Minamide R, Sato A, Yoshitake T, Takeda A, Egusa H, Tsuji T.
Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.
Sci Adv
2016
2:e1500887
PubMed ID: 27051874
DOI: 10.1126/sciadv.1500887
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9741
Tanaka T, Yokoi T, Tamalu F, Watanabe S, Nishina S, Azuma N.
Generation of Retinal Ganglion Cells With Functional Axons From Mouse Embryonic Stem Cells and Induced Pluripotent Stem Cells.
Invest. Ophthalmol. Vis. Sci.
2016
57:3348-59
PubMed ID: 27367502
DOI: 10.1167/iovs.16-19166
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12288
Kung-Kai Kuo, King-Teh Lee, Ker-Kong Chen, Ya-Han Yang, Ying-Chu Lin, Ming-Ho Tsai, Kenly Wuputra, Yen-Liang Lee, Chia-Chen Ku, Hiroyuki Miyoshi, Yukio Nakamura, Shigeo Saito, Chun-Chieh Wu, Chee-Yin Chai, Richard Eckner, Chen-Lung Steve Lin, Sophie S-W Wang, Deng-Chyang Wu, Chang-Shen Lin, Kazunari K Yokoyama
Positive Feedback Loop of OCT4 and c-JUN Expedites Cancer Stemness in Liver Cancer
Stem Cells
2016
34(11):2613-2624
PubMed ID: 27341307
DOI: 10.1002/stem.2447
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