Cell No. : Cell Name
APS0005 : iPS-Stm-FB/gfp-99-1
update : 2024/08/14
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| Comment | Mouse iPS cell line derived from stomach cells. Expressing GFP by CAG promoter. βgeo is knocked-in at Fbx15 locus.Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0033.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Originator |
Yamanaka, Shinya
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| Year of deposit |
2009
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| Animal |
_mouse
< Mammals
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| Gender |
Male
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| Age at sampling |
12 weeks
|
| Tissue |
stomach
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
pMXs, Oct3/4, Sox2, Klf4, c-Myc, GFP,βgeo gene
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|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_T789
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 6 cells/100 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
|
OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
6
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| User's Publication |
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14065
Yokoi T, Uemura T, Takamatsu K, Shintani K, Onode E, Hama S, Miyashima Y, Okada M, Nakamura H.
Fate and contribution of induced pluripotent stem cell-derived neurospheres transplanted with nerve conduits to promoting peripheral nerve regeneration in mice
Biomed Mater Eng
2021
Mar 23
PubMed ID: 33780359
DOI: 10.3233/BME-201182
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17106
Nhien Thi Nguyen, Manita Wittayarat, Zhao Namula, Yoko Sato, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara, Maki Hirata, Takeshige Otoi
Chlorogenic acid and insulin–transferrin–selenium supplementation during in vitro maturation enhances the developmental competence of interspecies chimera blastocysts following cell injection
Challenges
2021
49:486-491
DOI: 10.1080/09712119.2021.2011295
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10837
Iida S, Shimba K, Sakai K, Kotani K, Jimbo Y.
Synchronous firing patterns of induced pluripotent stem cell-derived cortical neurons depend on the network structure consisting of excitatory and inhibitory neurons.
Biochem. Biophys. Res. Commun.
2018
501:152-157
PubMed ID: 29723524
DOI: 10.1016/j.bbrc.2018.04.197
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11029
Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells
Journal of Hard Tissue Biology
2018
27, 1-10
DOI: 10.2485/jhtb.27.1
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7075
Takagi R, Ishimaru J, Sugawara A, Toyoshima KE, Ishida K, Ogawa M, Sakakibara K, Asakawa K, Kashiwakura A, Oshima M, Minamide R, Sato A, Yoshitake T, Takeda A, Egusa H, Tsuji T.
Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.
Sci Adv
2016
2:e1500887
PubMed ID: 27051874
DOI: 10.1126/sciadv.1500887
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14083
Iida Shoko, Shimba Kenta, Kotani Kiyoshi, Jimbo Yasuhiko
Different Activity Patterns in Cortical Network of iPS Cell-derived Excitatory/Inhibitory Neurons
Frontiers in Neuroscience
2016
10
DOI: 10.3389/conf.fnins.2016.93.00059
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