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Cell No. : Cell Name
APS0005 : iPS-Stm-FB/gfp-99-1  update : 2023/02/02
CommentMouse iPS cell line derived from stomach cells. Expressing GFP by CAG promoter. βgeo is knocked-in at Fbx15 locus.Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read Announcement of GFP bioresources 3) Attach Appendix to MTA.
Remarks
Order Form Order Form(C-0033.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor https://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2009
Animal mouse < Mammals
Gender Male
Age at sampling 12 weeks
Tissue stomach
Classification iPS
Recombinant recombinant
Exogene pMXs, Oct3/4, Sox2, Klf4, c-Myc, GFP,βgeo gene
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T789
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 6


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Reference
3674  Aoi T, Yae K, Nakagawa M, Ichisaka T, Okita K, Takahashi K, Chiba T, Yamanaka S.  Generation of pluripotent stem cells from adult mouse liver and stomach cells.  Science  2008  321(5889):699-702  PubMed ID: 18276851   DOI: 10.1126/science.1154884

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User's Publication
14065  Yokoi T, Uemura T, Takamatsu K, Shintani K, Onode E, Hama S, Miyashima Y, Okada M, Nakamura H.  Fate and contribution of induced pluripotent stem cell-derived neurospheres transplanted with nerve conduits to promoting peripheral nerve regeneration in mice  Biomed Mater Eng  2021  Mar 23  PubMed ID: 33780359   DOI: 10.3233/BME-201182
17106  Nhien Thi Nguyen, Manita Wittayarat, Zhao Namula, Yoko Sato, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Fuminori Tanihara, Maki Hirata, Takeshige Otoi  Chlorogenic acid and insulin–transferrin–selenium supplementation during in vitro maturation enhances the developmental competence of interspecies chimera blastocysts following cell injection  Challenges  2021  49:486-491    DOI: 10.1080/09712119.2021.2011295
10837  Iida S, Shimba K, Sakai K, Kotani K, Jimbo Y.  Synchronous firing patterns of induced pluripotent stem cell-derived cortical neurons depend on the network structure consisting of excitatory and inhibitory neurons.  Biochem. Biophys. Res. Commun.  2018  501:152-157  PubMed ID: 29723524   DOI: 10.1016/j.bbrc.2018.04.197
11029    Craniofacial Bone Regeneration using iPS Cell-Derived Neural Crest Like Cells  Journal of Hard Tissue Biology  2018  27, 1-10    DOI: 10.2485/jhtb.27.1
7075  Takagi R, Ishimaru J, Sugawara A, Toyoshima KE, Ishida K, Ogawa M, Sakakibara K, Asakawa K, Kashiwakura A, Oshima M, Minamide R, Sato A, Yoshitake T, Takeda A, Egusa H, Tsuji T.  Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model.  Sci Adv  2016  2:e1500887  PubMed ID: 27051874   DOI: 10.1126/sciadv.1500887
14083  Iida Shoko, Shimba Kenta, Kotani Kiyoshi, Jimbo Yasuhiko  Different Activity Patterns in Cortical Network of iPS Cell-derived Excitatory/Inhibitory Neurons  Frontiers in Neuroscience  2016  10    DOI: 10.3389/conf.fnins.2016.93.00059



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