Cell No. : Cell Name
APS0004 : iPS-MEF-Ng-492B-4
update : 2024/11/11
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| Comment | Mouse iPS cell line established without retrovirus vector. It has no integration of exogene. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA . |
| Remarks | |
| Order Form |
Order Form(C-0033.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Originator |
Yamanaka, Shinya
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| Year of deposit |
2008
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| Animal |
_mouse
< Mammals
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| Gender |
Male
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| Age at sampling |
embryo
|
| Tissue |
embryonic fibroblast
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, c-Myc, Nanog-GFP-IRES-Puro
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_T788
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 6 cells/100 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Mycoplasma |
(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
9
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| User's Publication |
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21474
Nakajima T, Imai A, Ishii C, Tsuruyama K, Yamanaka R, Tomooka Y, Saito S, Adachi N, Kohno S, Sato T.
SMAD2/3 signaling regulates initiation of mouse Wolffian ducts and proximal differentiation in Müllerian ducts.
FEBS Open Bio
2023
PubMed ID: 37953493
DOI: 10.1002/2211-5463.13729
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13086
Kenmochi H, Yamasaki T, Koizumi S, Sameshima T, Namba H.
Nicotine does not affect stem cell properties requisite for suicide gene therapy against glioma.
Neurol Res
2020
PubMed ID: 32588772
DOI: 10.1080/01616412.2020.1782123
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13111
Kawakita N, Toba H, Miyoshi K, Sakamoto S, Matsumoto D, Takashima M, Aoyama M, Inoue S, Morimoto M, Nishino T, Takizawa H, Tangoku A.
Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration.
Stem Cell Res Ther
2020
PubMed ID: 33008488
DOI: 10.1186/s13287-020-01946-7
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13521
Tao F, Wang F, Zhang W, Hao Y.
MicroRNA-22 enhances the differentiation of mouse induced pluripotent stem cells into alveolar epithelial type II cells
Eur J Histochem
2020
64(s2):3170
PubMed ID: 33334090
DOI: 10.4081/ejh.2020.3170
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13589
Hosoyama T.
Possible application of muscle specific conditional mouse-derived induced pluripotent stem cells for muscle research
Biochem Biophys Rep
2020
21:100744
PubMed ID: 32025579
DOI: 10.1016/j.bbrep.2020.100744
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11163
Zhou Q, Ye X, Ran Q, Kitahara A, Matsumoto Y, Moriyama M, Ajioka Y, Saijo Y.
Trachea Engineering Using a Centrifugation Method and Mouse-Induced Pluripotent Stem Cells.
Tissue Eng Part C Methods
2018
24(9):524-533
PubMed ID: 30101671
DOI: 10.1089/ten.TEC.2018.0115
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16015
Zhou Q, Ye X, Sun R, Matsumoto Y, Moriyama M, Asano Y, Ajioka Y, Saijo Y.
Differentiation of mouse induced pluripotent stem cells into alveolar epithelial cells in vitro for use in vivo
Stem Cells Transl Med
2014
3(6):675-85
PubMed ID: 24763685
DOI: 10.5966/sctm.2013-0142
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8063
Yamashita T, Kawai H, Tian F, Ohta Y, Abe K.
Tumorigenic development of induced pluripotent stem cells in ischemic mouse brain.
Cell Transplant
2011
20:883-91
PubMed ID: 21054935
DOI: 10.3727/096368910X539092
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15538
Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M.
Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells
Cell
2011
146(4):519-32
PubMed ID: 21820164
DOI: 10.1016/j.cell.2011.06.052
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