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Cell No. : Cell Name
APS0004 : iPS-MEF-Ng-492B-4  update : 2022/05/27
CommentMouse iPS cell line established without retrovirus vector. It has no integration of exogene. Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read Announcement of GFP bioresources 3) Attach TERMS and CONDITIONS to MTA .
Remarks
Order Form Order Form(C-0033.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor https://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2008
Animal mouse < Mammals
Gender Male
Age at sampling embryo
Tissue embryonic fibroblast
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc, Nanog-GFP-IRES-Puro
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T788
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 8


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Reference
4558  Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S.  Generation of mouse induced pluripotent stem cells without viral vectors.  Science  2008  322:949-953  PubMed ID: 18845712

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User's Publication
13521  Tao F, Wang F, Zhang W, Hao Y.  MicroRNA-22 enhances the differentiation of mouse induced pluripotent stem cells into alveolar epithelial type II cells  Eur J Histochem  2020   64(s2):3170  PubMed ID: 33334090
13086  Kenmochi H, Yamasaki T, Koizumi S, Sameshima T, Namba H.  Nicotine does not affect stem cell properties requisite for suicide gene therapy against glioma.  Neurol Res  2020    PubMed ID: 32588772
13111  Kawakita N, Toba H, Miyoshi K, Sakamoto S, Matsumoto D, Takashima M, Aoyama M, Inoue S, Morimoto M, Nishino T, Takizawa H, Tangoku A.  Bronchioalveolar stem cells derived from mouse-induced pluripotent stem cells promote airway epithelium regeneration.  Stem Cell Res Ther  2020    PubMed ID: 33008488
13589  Hosoyama T.  Possible application of muscle specific conditional mouse-derived induced pluripotent stem cells for muscle research  Biochem Biophys Rep  2020  21:100744  PubMed ID: 32025579
11163  Zhou Q, Ye X, Ran Q, Kitahara A, Matsumoto Y, Moriyama M, Ajioka Y, Saijo Y.  Trachea Engineering Using a Centrifugation Method and Mouse-Induced Pluripotent Stem Cells.  Tissue Eng Part C Methods  2018  24(9):524-533  PubMed ID: 30101671
16015  Zhou Q, Ye X, Sun R, Matsumoto Y, Moriyama M, Asano Y, Ajioka Y, Saijo Y.  Differentiation of mouse induced pluripotent stem cells into alveolar epithelial cells in vitro for use in vivo  Stem Cells Transl Med  2014  3(6):675-85  PubMed ID: 24763685
8063  Yamashita T, Kawai H, Tian F, Ohta Y, Abe K.  Tumorigenic development of induced pluripotent stem cells in ischemic mouse brain.  Cell Transplant  2011  20:883-91  PubMed ID: 21054935
15538  Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M.  Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells  Cell  2011  146(4):519-32  PubMed ID: 21820164



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