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Cell No. : Cell Name
APS0003 : iPS-MEF-Fb/Ng-440A-3  update : 2023/07/19
CommentMouse iPS cell line established without retrovirus vector. It has no integration of exogene. Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 3) Attach Appendix to MTA.
Order Form Order Form(C-0033.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2008
Animal _mouse < Mammals
Gender Female
Age at sampling embryo
Tissue embryonic fibroblast
Classification iPS
Recombinant recombinant
Exogene Oct3/4, Sox2, Klf4, c-Myc, Fbx15βgeo/βgeo, Nanog-GFP-IRES-Puro
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T785
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10 6 cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 6

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4558  Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S.  Generation of mouse induced pluripotent stem cells without viral vectors.  Science  2008  322:949-953  PubMed ID: 18845712   DOI: 10.1126/science.1164270

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User's Publication
17634  Morii A, Inazu T.  HDAC8 is implicated in embryoid body formation via canonical Hedgehog signaling and regulates neuronal differentiation  Biochem Biophys Res Commun  2022  629:78-85  PubMed ID: 36113181   DOI: 10.1016/j.bbrc.2022.08.068
15512  Kurata A, Takanashi M, Ohno SI, Fujita K, Kuroda M.  Cisplatin induces differentiation in teratomas derived from pluripotent stem cells  Regen Ther  2021  18:117-126  PubMed ID: 34141836   DOI: 10.1016/j.reth.2021.05.005
4405  Jiayin Fu, Yon Jin Chuah, Jian Liu, Si Ying Tan, and Dong-An Wang  Respective Effects of Gelatin-Coated Polydimethylsiloxane (PDMS) Substrates on Self-renewal and Cardiac Differentiation of Induced Pluripotent Stem Cells (iPSCs)  ACS Biomater. Sci. Eng  2018  4:4321-4330  PubMed ID: 33418827   DOI: 10.1021/acsbiomaterials.8b00993
9572  He P, Fu J, Wang DA.  Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform.  Acta Biomater  2016  35:87-97  PubMed ID: 26911880   DOI: 10.1016/j.actbio.2016.02.026
9757  Higuchi S, Yoshina S, Mitani S.  Inhibition of the integrin signal constitutes a mouse iPS cell niche.  Dev. Growth Differ.  2016  58:586-99  PubMed ID: 27633818   DOI: 10.1111/dgd.12302
9932  Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.  In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.  Stem Cell Reports  2016  7:1023-1036  PubMed ID: 27840044   DOI: 10.1016/j.stemcr.2016.10.005

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