Cell No. : Cell Name
APS0003 : iPS-MEF-Fb/Ng-440A-3
update : 2023/07/19
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| Comment | Mouse iPS cell line established without retrovirus vector. It has no integration of exogene. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 3) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0033.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Originator |
Yamanaka, Shinya
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| Year of deposit |
2008
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| Animal |
_mouse
< Mammals
|
| Gender |
Female
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| Age at sampling |
embryo
|
| Tissue |
embryonic fibroblast
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
Oct3/4, Sox2, Klf4, c-Myc, Fbx15βgeo/βgeo, Nanog-GFP-IRES-Puro
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|
Lifespan |
infinite
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| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_T785
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
|
Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 6 cells/100 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
6
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| User's Publication |
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17634
Morii A, Inazu T.
HDAC8 is implicated in embryoid body formation via canonical Hedgehog signaling and regulates neuronal differentiation
Biochem Biophys Res Commun
2022
629:78-85
PubMed ID: 36113181
DOI: 10.1016/j.bbrc.2022.08.068
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15512
Kurata A, Takanashi M, Ohno SI, Fujita K, Kuroda M.
Cisplatin induces differentiation in teratomas derived from pluripotent stem cells
Regen Ther
2021
18:117-126
PubMed ID: 34141836
DOI: 10.1016/j.reth.2021.05.005
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4405
Jiayin Fu, Yon Jin Chuah, Jian Liu, Si Ying Tan, and Dong-An Wang
Respective Effects of Gelatin-Coated Polydimethylsiloxane (PDMS) Substrates on Self-renewal and Cardiac Differentiation of Induced Pluripotent Stem Cells (iPSCs)
ACS Biomater. Sci. Eng
2018
4:4321-4330
PubMed ID: 33418827
DOI: 10.1021/acsbiomaterials.8b00993
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9572
He P, Fu J, Wang DA.
Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform.
Acta Biomater
2016
35:87-97
PubMed ID: 26911880
DOI: 10.1016/j.actbio.2016.02.026
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9757
Higuchi S, Yoshina S, Mitani S.
Inhibition of the integrin signal constitutes a mouse iPS cell niche.
Dev. Growth Differ.
2016
58:586-99
PubMed ID: 27633818
DOI: 10.1111/dgd.12302
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9932
Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.
In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.
Stem Cell Reports
2016
7:1023-1036
PubMed ID: 27840044
DOI: 10.1016/j.stemcr.2016.10.005
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