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細胞番号 : 細胞名
APS0002 : iPS-MEF-Ng-178B-5  update : 2021/05/07
細胞特性(Comment:英)Mouse iPS cell line established with three factors, Oct3/4, Sox2 and Klf4, using retrovirus vector. Order Form ( C-0033, C-00034). Please see CiRA Foundation, if you are a researcher at a for-profit organization.
細胞特性(日)マウスiPS細胞。Oct3/4, Sox2, Klf4の3因子のみで樹立された株。レトロウイルスベクター使用。営利機関は、 公益財団法人京都大学iPS細胞研究財団から配布。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 3) Attach TERMS and CONDITIONS to MTA (C-0034) .
使用条件(日)1) 利用者は学術機関に所属している者に限る。2) GE Healthcare Bio-Sciences社とBRCとのGFP Transfer License 締結 を確認の上、手続きを取ること。 3) 別紙1を提供同意書 (C-0028) に添付すること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0033.pdf)   MTA(C-0034.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0027.pdf   同意書C-0028.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 http://www.cira.kyoto-u.ac.jp/
樹立者 Yamanaka, Shinya
寄託日 2008
動物種 _mouse < Mammals
性別 Male
embryo
採取組織 embryonic fibroblast
細胞分類 iPS
遺伝子改変 recombinant
外来遺伝子 pMXs,Oct3/4, Sox2, Klf4, Nanog-GFP-IRES-Puro^r
細胞寿命 infinite
細胞形態 ES-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
抗生物質 Free
継代方法 0.25% trypsin + 0.02% EDTA
培養容器のコーティング 0.1% gelatin coated dish
播種細胞数 2 x 10^(6) cells/100 mm dish
継代・培地交換頻度 Subculture : once/2-3 days, Medium Renewal : every day
培養最適温度 37 ℃
二酸化炭素濃度 5 %
フィーダー細胞 SNL 76/7
フィーダー細胞の処理方法 X-rays 5000R (or MMC)
フィーダー細胞の播種数 1-2 x 10^(6) cells/100 mm dish
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 1件
Reference(日) 0件
利用者成果(英) 20件
利用者成果(日) 0件

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Reference(英)
3673  Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S.  Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.  Nat Biotechnol  2008  26(1):101-6  PubMed ID: 18059259

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Reference(日)

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利用者成果(英)
14064  Yokoi T, Uemura T, Takamatsu K, Onode E, Shintani K, Hama S, Miyashima Y, Okada M, Nakamura H.  Long-term survival of transplanted induced pluripotent stem cell-derived neurospheres with nerve conduit into sciatic nerve defects in immunosuppressed mice  Biochem Biophys Rep  2021  26:100979  PubMed ID: 33817351
12084  Sharmin A, Adnan N, Haque A, Mashimo Y, Mie M, Kobatake E.  Construction of multifunctional fusion proteins with a laminin-derived short peptide to promote neural differentiation of mouse induced pluripotent stem cells.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2020    PubMed ID: 32167675
11644  Ser-Od T, Al-Wahabi A, Inoue K, Nakajima K, Matsuzaka K, Inoue T.  Effect of EDTA-treated dentin on the differentiation of mouse iPS cells into osteogenic/odontogenic lineages in vitro and in vivo.  Dent Mater J  2019    PubMed ID: 31341145
10438  Yusuke Kambe, Takayuki Tokushige, Atsushi Mahara, Yasuhiko Iwasaki & Tetsuji Yamaoka  Cardiac differentiation of induced pluripotent stem cells on elastin-like protein-based hydrogels presenting a single-cell adhesion sequence  Polymer Journal  2019  51:97–105 
10507  Yokoi T, Uemura T, Takamatsu K, Shintani K, Onode E, Okada M, Hidaka N, Nakamura H.  Bioabsorbable nerve conduits coated with induced pluripotent stem cell-derived neurospheres enhance axonal regeneration in sciatic nerve defects in aged mice.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2018  106(5):1752-1758  PubMed ID: 28888079
10567  Hirata M, Yamaoka T.  Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of induced pluripotent stem (iPS) cells at different stages.  Acta Biomater  2018  65:44-52  PubMed ID: 29066419
10078  Otaka A, Kitagawa K, Nakaoki T, Hirata M, Fukazawa K, Ishihara K, Mahara A, Yamaoka T.  Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel.  Langmuir  2017  33:1576-1582  PubMed ID: 28092949
10454  Hirata M, Yamaoka T.  Hepatocytic differentiation of iPS cells on decellularized liver tissue.  J Artif Organs  2017  20:318-325  PubMed ID: 28776092
10472  Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.  Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.  Proc. Natl. Acad. Sci. U.S.A.  2017  114:E7479-E7488  PubMed ID: 28827343
9757  Higuchi S, Yoshina S, Mitani S.  Inhibition of the integrin signal constitutes a mouse iPS cell niche.  Dev. Growth Differ.  2016  58:586-99  PubMed ID: 27633818
9932  Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.  In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.  Stem Cell Reports  2016  7:1023-1036  PubMed ID: 27840044
6225  Sugimoto K, Hayashi Y2.  The Characteristics of Murine iPS Cells and siRNA Transfection Under Hypoxia.  Methods Mol Biol  2016  1357:465-74  PubMed ID: 25476444
6227  Yoshida K, Sato J, Takai R, Uehara O, Kurashige Y, Nishimura M, Chiba I, Saitoh M, Abiko Y  Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.  Med Mol Morphol  2015  48(3):138-45  PubMed ID: 25319805
7418  Uemura T, Ikeda M, Takamatsu K, Yokoi T, Okada M, Nakamura H.  Long-term efficacy and safety outcomes of transplantation of induced pluripotent stem cell-derived neurospheres with bioabsorbable nerve conduits for peripheral nerve regeneration in mice.  Cells Tissues Organs (Print)  2014  200:78-91  PubMed ID: 25823624
6228  Ikeda M, Uemura T, Takamatsu K, Okada M, Kazuki K, Tabata Y, Ikada Y, Nakamura H.  Acceleration of peripheral nerve regeneration using nerve conduits in combination with induced pluripotent stem cell technology and a basic fibroblast growth factor drug delivery system.  J Biomed Mater Res A  2014  102(5):1370-8  PubMed ID: 23733515
6202  Sugimoto K, Yoshizawa Y, Yamada S, Igawa K, Hayashi Y, Ishizaki H.  Effects of hypoxia on pluripotency in murine iPS cells.  Microsc Res Tech  2013  76(10):1084-92  PubMed ID: 23878105
7664  Takeuchi F, Yokota M, Yamamoto K, Nakashima E, Katsuya T, Asano H, Isono M, Nabika T, Sugiyama T, Fujioka A, Awata N, Ohnaka K, Nakatochi M, Kitajima H, Rakugi H, Nakamura J, Ohkubo T, Imai Y, Shimamoto K, Yamori Y, Yamaguchi S, Kobayashi S, Takayanagi R, Ogihara T, Kato N.  Genome-wide association study of coronary artery disease in the Japanese.  Eur. J. Hum. Genet.  2012  20:333-40  PubMed ID: 21971053
6223  Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.  Transplantation of induced pluripotent stem cell-derived neurospheres for peripheral nerve repair.  Biochem Biophys Res Commun  2012  419(1):130-5  PubMed ID: 22333572
7766  Kawagoe S, Higuchi T, Meng XL, Shimada Y, Shimizu H, Hirayama R, Fukuda T, Chang H, Nakahata T, Fukada S, Ida H, Kobayashi H, Ohashi T, Eto Y.  Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells.  Mol. Genet. Metab.  2011  104:123-8  PubMed ID: 21703893
6224  Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.  A tissue-engineered bioabsorbable nerve conduit created by three-dimensional culture of induced pluripotent stem cell-derived neurospheres.  Biomed Mater Eng  2011  21(5-6):333-9  PubMed ID: 22561252

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