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Cell No. : Cell Name
APS0002 : iPS-MEF-Ng-178B-5  update : 2023/07/19
CommentMouse iPS cell line established with three factors, Oct3/4, Sox2 and Klf4, using retrovirus vector. Please see CiRA Foundation, if you are a researcher at a for-profit organization.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA.
Order Form Order Form(C-0033.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor
Originator Yamanaka, Shinya
Year of deposit 2008
Animal _mouse < Mammals
Gender Male
Age at sampling embryo
Tissue embryonic fibroblast
Classification iPS
Recombinant recombinant
Exogene pMXs,Oct3/4, Sox2, Klf4, Nanog-GFP-IRES-Puro^r
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_T786
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10 6 cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 23

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3673  Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, Okita K, Mochiduki Y, Takizawa N, Yamanaka S.  Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.  Nat Biotechnol  2008  26(1):101-6  PubMed ID: 18059259   DOI: 10.1038/nbt1374

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User's Publication
14064  Yokoi T, Uemura T, Takamatsu K, Onode E, Shintani K, Hama S, Miyashima Y, Okada M, Nakamura H.  Long-term survival of transplanted induced pluripotent stem cell-derived neurospheres with nerve conduit into sciatic nerve defects in immunosuppressed mice  Biochem Biophys Rep  2021  26:100979  PubMed ID: 33817351   DOI: 10.1016/j.bbrep.2021.100979
14695  Akihisa Otaka, Atsushi Mahara, Kazuhiko Ishihara, Tetsuji Yamaoka  Adhesion of Flk1-expressing cells under shear flow in phospholipid polymer-coated immunoaffinity channels  Journal of Micromechanics and Microengineering  2021  31:045012    DOI: 10.1088/1361-6439/abe52a
12084  Sharmin A, Adnan N, Haque A, Mashimo Y, Mie M, Kobatake E.  Construction of multifunctional fusion proteins with a laminin-derived short peptide to promote neural differentiation of mouse induced pluripotent stem cells.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2020    PubMed ID: 32167675   DOI: 10.1002/jbm.b.34600
11644  Ser-Od T, Al-Wahabi A, Inoue K, Nakajima K, Matsuzaka K, Inoue T.  Effect of EDTA-treated dentin on the differentiation of mouse iPS cells into osteogenic/odontogenic lineages in vitro and in vivo.  Dent Mater J  2019    PubMed ID: 31341145   DOI: 10.4012/dmj.2018-161
10438  Yusuke Kambe, Takayuki Tokushige, Atsushi Mahara, Yasuhiko Iwasaki & Tetsuji Yamaoka  Cardiac differentiation of induced pluripotent stem cells on elastin-like protein-based hydrogels presenting a single-cell adhesion sequence  Polymer Journal  2019  51:97–105    DOI: 10.1038/s41428-018-0110-2
10507  Yokoi T, Uemura T, Takamatsu K, Shintani K, Onode E, Okada M, Hidaka N, Nakamura H.  Bioabsorbable nerve conduits coated with induced pluripotent stem cell-derived neurospheres enhance axonal regeneration in sciatic nerve defects in aged mice.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2018  106(5):1752-1758  PubMed ID: 28888079   DOI: 10.1002/jbm.b.33983
10567  Hirata M, Yamaoka T.  Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of induced pluripotent stem (iPS) cells at different stages.  Acta Biomater  2018  65:44-52  PubMed ID: 29066419   DOI: 10.1016/j.actbio.2017.10.032
10078  Otaka A, Kitagawa K, Nakaoki T, Hirata M, Fukazawa K, Ishihara K, Mahara A, Yamaoka T.  Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel.  Langmuir  2017  33:1576-1582  PubMed ID: 28092949   DOI: 10.1021/acs.langmuir.6b04070
10454  Hirata M, Yamaoka T.  Hepatocytic differentiation of iPS cells on decellularized liver tissue.  J Artif Organs  2017  20:318-325  PubMed ID: 28776092   DOI: 10.1007/s10047-017-0977-2
10472  Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.  Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.  Proc. Natl. Acad. Sci. U.S.A.  2017  114:E7479-E7488  PubMed ID: 28827343   DOI: 10.1073/pnas.1703170114
9757  Higuchi S, Yoshina S, Mitani S.  Inhibition of the integrin signal constitutes a mouse iPS cell niche.  Dev. Growth Differ.  2016  58:586-99  PubMed ID: 27633818   DOI: 10.1111/dgd.12302
9932  Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.  In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.  Stem Cell Reports  2016  7:1023-1036  PubMed ID: 27840044   DOI: 10.1016/j.stemcr.2016.10.005
6225  Sugimoto K, Hayashi Y2.  The Characteristics of Murine iPS Cells and siRNA Transfection Under Hypoxia.  Methods Mol Biol  2016  1357:465-74  PubMed ID: 25476444   DOI: 10.1007/7651_2014_158
14576  Adnan N, Mie M, Haque A, Hossain S, Mashimo Y, Akaike T, Kobatake E.  Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells  Bioconjug Chem  2016  27(7):1599-605  PubMed ID: 27269811   DOI: 10.1021/acs.bioconjchem.6b00141
6227  Yoshida K, Sato J, Takai R, Uehara O, Kurashige Y, Nishimura M, Chiba I, Saitoh M, Abiko Y  Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.  Med Mol Morphol  2015  48(3):138-45  PubMed ID: 25319805   DOI: 10.1007/s00795-014-0088-6
7418  Uemura T, Ikeda M, Takamatsu K, Yokoi T, Okada M, Nakamura H.  Long-term efficacy and safety outcomes of transplantation of induced pluripotent stem cell-derived neurospheres with bioabsorbable nerve conduits for peripheral nerve regeneration in mice.  Cells Tissues Organs (Print)  2014  200:78-91  PubMed ID: 25823624   DOI: 10.1159/000370322
6228  Ikeda M, Uemura T, Takamatsu K, Okada M, Kazuki K, Tabata Y, Ikada Y, Nakamura H.  Acceleration of peripheral nerve regeneration using nerve conduits in combination with induced pluripotent stem cell technology and a basic fibroblast growth factor drug delivery system.  J Biomed Mater Res A  2014  102(5):1370-8  PubMed ID: 23733515   DOI: 10.1002/jbm.a.34816
15806  Michiyo Terashimaa, Maho Amanob, Tomohiro Onoderaa, Shin-Ichiro Nishimurab, Norimasa Iwasakia,  Potential for Tumorigenesis and Repair of Osteochondral Defects by iPS Cell Transplantation in Rat  Stem Cell Research  2014  13:454-464   
6202  Sugimoto K, Yoshizawa Y, Yamada S, Igawa K, Hayashi Y, Ishizaki H.  Effects of hypoxia on pluripotency in murine iPS cells.  Microsc Res Tech  2013  76(10):1084-92  PubMed ID: 23878105   DOI: 10.1002/jemt.22269
6223  Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.  Transplantation of induced pluripotent stem cell-derived neurospheres for peripheral nerve repair.  Biochem Biophys Res Commun  2012  419(1):130-5  PubMed ID: 22333572   DOI: 10.1016/j.bbrc.2012.01.154
7766  Kawagoe S, Higuchi T, Meng XL, Shimada Y, Shimizu H, Hirayama R, Fukuda T, Chang H, Nakahata T, Fukada S, Ida H, Kobayashi H, Ohashi T, Eto Y.  Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells.  Mol. Genet. Metab.  2011  104:123-8  PubMed ID: 21703893   DOI: 10.1016/j.ymgme.2011.05.020
6224  Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.  A tissue-engineered bioabsorbable nerve conduit created by three-dimensional culture of induced pluripotent stem cell-derived neurospheres.  Biomed Mater Eng  2011  21(5-6):333-9  PubMed ID: 22561252   DOI: 10.3233/BME-2012-0680
15538  Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M.  Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells  Cell  2011  146(4):519-32  PubMed ID: 21820164   DOI: 10.1016/j.cell.2011.06.052

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