Cell No. : Cell Name
APS0002 : iPS-MEF-Ng-178B-5
update : 2025/01/16
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| Comment | Mouse iPS cell line established with three factors, Oct3/4, Sox2 and Klf4, using retrovirus vector. Please see CiRA Foundation, if you are a researcher at a for-profit organization. |
| Comment from the depositor | |
| Terms and conditions | 1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach Appendix to MTA. |
| Remarks | |
| Order Form |
Order Form(C-0033.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
https://www.cira.kyoto-u.ac.jp/
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| Originator |
Yamanaka, Shinya
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| Year of deposit |
2008
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| Animal |
_mouse
< Mammals
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic fibroblast
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| Classification |
iPS
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| Recombinant |
recombinant
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| Exogene |
pMXs,Oct3/4, Sox2, Klf4, Nanog-GFP-IRES-Puro^r
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_T786
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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SNL 76/7
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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1-2 x 10 6 cells/100 mm dish
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Mycoplasma |
(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
23
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| User's Publication |
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14064
Yokoi T, Uemura T, Takamatsu K, Onode E, Shintani K, Hama S, Miyashima Y, Okada M, Nakamura H.
Long-term survival of transplanted induced pluripotent stem cell-derived neurospheres with nerve conduit into sciatic nerve defects in immunosuppressed mice
Biochem Biophys Rep
2021
26:100979
PubMed ID: 33817351
DOI: 10.1016/j.bbrep.2021.100979
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14695
Akihisa Otaka, Atsushi Mahara, Kazuhiko Ishihara, Tetsuji Yamaoka
Adhesion of Flk1-expressing cells under shear flow in phospholipid polymer-coated immunoaffinity channels
Journal of Micromechanics and Microengineering
2021
31:045012
DOI: 10.1088/1361-6439/abe52a
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12084
Sharmin A, Adnan N, Haque A, Mashimo Y, Mie M, Kobatake E.
Construction of multifunctional fusion proteins with a laminin-derived short peptide to promote neural differentiation of mouse induced pluripotent stem cells.
J. Biomed. Mater. Res. Part B Appl. Biomater.
2020
PubMed ID: 32167675
DOI: 10.1002/jbm.b.34600
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11644
Ser-Od T, Al-Wahabi A, Inoue K, Nakajima K, Matsuzaka K, Inoue T.
Effect of EDTA-treated dentin on the differentiation of mouse iPS cells into osteogenic/odontogenic lineages in vitro and in vivo.
Dent Mater J
2019
PubMed ID: 31341145
DOI: 10.4012/dmj.2018-161
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10438
Yusuke Kambe, Takayuki Tokushige, Atsushi Mahara, Yasuhiko Iwasaki & Tetsuji Yamaoka
Cardiac differentiation of induced pluripotent stem cells on elastin-like protein-based hydrogels presenting a single-cell adhesion sequence
Polymer Journal
2019
51:97–105
DOI: 10.1038/s41428-018-0110-2
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10507
Yokoi T, Uemura T, Takamatsu K, Shintani K, Onode E, Okada M, Hidaka N, Nakamura H.
Bioabsorbable nerve conduits coated with induced pluripotent stem cell-derived neurospheres enhance axonal regeneration in sciatic nerve defects in aged mice.
J. Biomed. Mater. Res. Part B Appl. Biomater.
2018
106(5):1752-1758
PubMed ID: 28888079
DOI: 10.1002/jbm.b.33983
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10567
Hirata M, Yamaoka T.
Effect of stem cell niche elasticity/ECM protein on the self-beating cardiomyocyte differentiation of induced pluripotent stem (iPS) cells at different stages.
Acta Biomater
2018
65:44-52
PubMed ID: 29066419
DOI: 10.1016/j.actbio.2017.10.032
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10078
Otaka A, Kitagawa K, Nakaoki T, Hirata M, Fukazawa K, Ishihara K, Mahara A, Yamaoka T.
Label-Free Separation of Induced Pluripotent Stem Cells with Anti-SSEA-1 Antibody Immobilized Microfluidic Channel.
Langmuir
2017
33:1576-1582
PubMed ID: 28092949
DOI: 10.1021/acs.langmuir.6b04070
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10454
Hirata M, Yamaoka T.
Hepatocytic differentiation of iPS cells on decellularized liver tissue.
J Artif Organs
2017
20:318-325
PubMed ID: 28776092
DOI: 10.1007/s10047-017-0977-2
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10472
Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.
Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.
Proc. Natl. Acad. Sci. U.S.A.
2017
114:E7479-E7488
PubMed ID: 28827343
DOI: 10.1073/pnas.1703170114
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9757
Higuchi S, Yoshina S, Mitani S.
Inhibition of the integrin signal constitutes a mouse iPS cell niche.
Dev. Growth Differ.
2016
58:586-99
PubMed ID: 27633818
DOI: 10.1111/dgd.12302
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9932
Fukunaga I, Fujimoto A, Hatakeyama K, Aoki T, Nishikawa A, Noda T, Minowa O, Kurebayashi N, Ikeda K, Kamiya K.
In Vitro Models of GJB2-Related Hearing Loss Recapitulate Ca2+ Transients via a Gap Junction Characteristic of Developing Cochlea.
Stem Cell Reports
2016
7:1023-1036
PubMed ID: 27840044
DOI: 10.1016/j.stemcr.2016.10.005
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6225
Sugimoto K, Hayashi Y2.
The Characteristics of Murine iPS Cells and siRNA Transfection Under Hypoxia.
Methods Mol Biol
2016
1357:465-74
PubMed ID: 25476444
DOI: 10.1007/7651_2014_158
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14576
Adnan N, Mie M, Haque A, Hossain S, Mashimo Y, Akaike T, Kobatake E.
Construction of a Defined Biomimetic Matrix for Long-Term Maintenance of Mouse Induced Pluripotent Stem Cells
Bioconjug Chem
2016
27(7):1599-605
PubMed ID: 27269811
DOI: 10.1021/acs.bioconjchem.6b00141
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6227
Yoshida K, Sato J, Takai R, Uehara O, Kurashige Y, Nishimura M, Chiba I, Saitoh M, Abiko Y
Differentiation of mouse iPS cells into ameloblast-like cells in cultures using medium conditioned by epithelial cell rests of Malassez and gelatin-coated dishes.
Med Mol Morphol
2015
48(3):138-45
PubMed ID: 25319805
DOI: 10.1007/s00795-014-0088-6
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7418
Uemura T, Ikeda M, Takamatsu K, Yokoi T, Okada M, Nakamura H.
Long-term efficacy and safety outcomes of transplantation of induced pluripotent stem cell-derived neurospheres with bioabsorbable nerve conduits for peripheral nerve regeneration in mice.
Cells Tissues Organs (Print)
2014
200:78-91
PubMed ID: 25823624
DOI: 10.1159/000370322
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6228
Ikeda M, Uemura T, Takamatsu K, Okada M, Kazuki K, Tabata Y, Ikada Y, Nakamura H.
Acceleration of peripheral nerve regeneration using nerve conduits in combination with induced pluripotent stem cell technology and a basic fibroblast growth factor drug delivery system.
J Biomed Mater Res A
2014
102(5):1370-8
PubMed ID: 23733515
DOI: 10.1002/jbm.a.34816
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15806
Michiyo Terashimaa, Maho Amanob, Tomohiro Onoderaa, Shin-Ichiro Nishimurab, Norimasa Iwasakia,
Potential for Tumorigenesis and Repair of Osteochondral Defects by iPS Cell Transplantation in Rat
Stem Cell Research
2014
13:454-464
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6202
Sugimoto K, Yoshizawa Y, Yamada S, Igawa K, Hayashi Y, Ishizaki H.
Effects of hypoxia on pluripotency in murine iPS cells.
Microsc Res Tech
2013
76(10):1084-92
PubMed ID: 23878105
DOI: 10.1002/jemt.22269
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6223
Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.
Transplantation of induced pluripotent stem cell-derived neurospheres for peripheral nerve repair.
Biochem Biophys Res Commun
2012
419(1):130-5
PubMed ID: 22333572
DOI: 10.1016/j.bbrc.2012.01.154
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7766
Kawagoe S, Higuchi T, Meng XL, Shimada Y, Shimizu H, Hirayama R, Fukuda T, Chang H, Nakahata T, Fukada S, Ida H, Kobayashi H, Ohashi T, Eto Y.
Generation of induced pluripotent stem (iPS) cells derived from a murine model of Pompe disease and differentiation of Pompe-iPS cells into skeletal muscle cells.
Mol. Genet. Metab.
2011
104:123-8
PubMed ID: 21703893
DOI: 10.1016/j.ymgme.2011.05.020
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6224
Uemura T, Takamatsu K, Ikeda M, Okada M, Kazuki K, Ikada Y, Nakamura H.
A tissue-engineered bioabsorbable nerve conduit created by three-dimensional culture of induced pluripotent stem cell-derived neurospheres.
Biomed Mater Eng
2011
21(5-6):333-9
PubMed ID: 22561252
DOI: 10.3233/BME-2012-0680
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15538
Hayashi K, Ohta H, Kurimoto K, Aramaki S, Saitou M.
Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells
Cell
2011
146(4):519-32
PubMed ID: 21820164
DOI: 10.1016/j.cell.2011.06.052
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