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Cell No. : Cell Name
AES1913 : 2TP-084  update : 2018/07/06
CommentThe Gene-trap Mouse ES cell (V6.4) clone. Misc_ID:unknown
Comment from the depositor
Terms and conditions1) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. When the RECIPIENT use the BIOLOGICAL RESOURCE in collaboration with a for-profit organization, it is recognized as a for-profit utilization and the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 3) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Dr. Yasumasa Ishida ) is requested. 5) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of a paper (Nucleic Acids Res. 2005;33:e20) is requested. 6) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 7) When the RECIPIENT established a mouse line by using of the BIOLOGICAL RESOURCE, the RECIPIENT must deposit the mouse line to the Experimental Animal Division of RIKEN BioResource Center.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ishida Yasumasa
Originator Ishida Yasumasa
Year of deposit 2011
Animal mouse < Mammals
Strain name C57BL/6J x 129/SvJae
Genus Mus
Species musculus
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene CTP2F
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 1-1.5 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells SNL 76/7
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1.5-2.5 x 10^(6) cells/100 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3667  Shigeoka T, Kawaichi M, Ishida Y.  Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells.  Nucleic Acids Res  2005  33(2):e20  PubMed ID: 15687378

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