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Cell No. : Cell Name
AES0204 : EpiSC 129Ba1  update : 2018/07/06
Comment
Comment from the depositorMouse Epiblast Stem cells (EpiSCs) established in the presence of Wnt secretion inhibitor, IWP-2. The cell line was derived from embryos obtained by 129S2/Sv x C57BL/6N cross. Homogeneous cellular state can be maintained in the culture medium containing IWP-2.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cell Reports 2015;4:744-757. ) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Abe, Kuniya
Originator Masayo Kondo, Michihiko Sugimoto, Kuniya Abe
Year of deposit 2015
Animal mouse < Mammals
Strain name 129S2/Sv x C57BL/6N hybrid
Gender Male
Age at sampling embryo
Tissue epiblast
Classification normal
Recombinant non-recombinant
Year of origin 2017
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 (2.5mM GlutaMAX) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 12ng/ml human basic FGF + 20ng/ml human Activin A + 2 μM IWP-2
Antibiotics Free
Passage method 0.25% trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 6-12 split
SC frequency Subculture : once/2 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
4600  Sugimoto M, Kondo M, Koga Y, Shiura H, Ikeda R, Hirose M, Ogura A, Murakami A, Yoshiki A, Chuva de Sousa Lopes SM, Abe K.  A simple and robust method for establishing homogeneous mouse epiblast stem cell lines by wnt inhibition.  Stem Cell Reports  2015  4(4):744-57  PubMed ID: 25818811

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User's Publication
12112  Matsuda M, Yamanaka Y, Uemura M, Osawa M, Saito MK, Nagahashi A, Nishio M, Guo L, Ikegawa S, Sakurai S, Kihara S, Maurissen TL, Nakamura M, Matsumoto T, Yoshitomi H, Ikeya M, Kawakami N, Yamamoto T, Woltjen K, Ebisuya M, Toguchida J, Alev C.  Recapitulating the human segmentation clock with pluripotent stem cells.  Nature  2020    PubMed ID: 32238941
11128  Kondo M, Sugimoto M, Abe K.  A Simplified and Efficient Protocol for Derivation and Maintenance of High-Quality Mouse Primed Pluripotent Stem Cells Using Wnt Inhibition.  Curr Protoc Stem Cell Biol  2018  46(1):e60  PubMed ID: 30005143



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