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Cell No. : Cell Name
AES0204 : EpiSC 129Ba1  update : 2023/01/06
Comment from the depositorMouse Epiblast Stem cells (EpiSCs) established in the presence of Wnt secretion inhibitor, IWP-2. The cell line was derived from embryos obtained by 129S2/Sv x C57BL/6N cross. Homogeneous cellular state can be maintained in the culture medium containing IWP-2.
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cell Reports 2015;4:744-757. ) designated by the DEPOSITOR is required.
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Abe, Kuniya
Originator Masayo Kondo, Michihiko Sugimoto, Kuniya Abe
Year of deposit 2015
Animal _mouse < Mammals
Strain name 129S2/Sv x C57BL/6N hybrid
Gender Male
Age at sampling embryo
Tissue epiblast
Classification normal
Recombinant non-recombinant
Year of origin 2017
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_C5QL
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2.5mM GlutaMAX) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 12ng/ml human basic FGF + 20ng/ml human Activin A + 2 μM IWP-2
Antibiotics Free
Passage method 0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 6-12 split
SC frequency Subculture : once/2 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10 5 cells/60 mm dish
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 4

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4600  Sugimoto M, Kondo M, Koga Y, Shiura H, Ikeda R, Hirose M, Ogura A, Murakami A, Yoshiki A, Chuva de Sousa Lopes SM, Abe K.  A simple and robust method for establishing homogeneous mouse epiblast stem cell lines by wnt inhibition.  Stem Cell Reports  2015  4(4):744-57  PubMed ID: 25818811   DOI: 10.1016/j.stemcr.2015.02.014

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User's Publication
19859  Lázaro J, Costanzo M, Sanaki-Matsumiya M, Girardot C, Hayashi M, Hayashi K, Diecke S, Hildebrandt TB, Lazzari G, Wu J, Petkov S, Behr R, Trivedi V, Matsuda M, Ebisuya M.  A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals  Cell Stem Cell  2023  30(7):938-949  PubMed ID: 37343565   DOI: 10.1016/j.stem.2023.05.014
13934  Ohhata T, Yamazawa K, Miura-Kamio A, Takahashi S, Sakai S, Tamura Y, Uchida C, Kitagawa K, Niida H, Hiratani I, Kobayashi H, Kimura H, Wutz A, Kitagawa M.  Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix  Cell Rep  2021  34(13):108912  PubMed ID: 33789104   DOI: 10.1016/j.celrep.2021.108912
12112  Matsuda M, Yamanaka Y, Uemura M, Osawa M, Saito MK, Nagahashi A, Nishio M, Guo L, Ikegawa S, Sakurai S, Kihara S, Maurissen TL, Nakamura M, Matsumoto T, Yoshitomi H, Ikeya M, Kawakami N, Yamamoto T, Woltjen K, Ebisuya M, Toguchida J, Alev C.  Recapitulating the human segmentation clock with pluripotent stem cells.  Nature  2020    PubMed ID: 32238941   DOI: 10.1038/s41586-020-2144-9
11128  Kondo M, Sugimoto M, Abe K.  A Simplified and Efficient Protocol for Derivation and Maintenance of High-Quality Mouse Primed Pluripotent Stem Cells Using Wnt Inhibition.  Curr Protoc Stem Cell Biol  2018  46(1):e60  PubMed ID: 30005143   DOI: 10.1002/cpsc.60

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