Cell No. : Cell Name
AES0204 : EpiSC 129Ba1
update : 2023/01/06
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| Comment | |
| Comment from the depositor | Mouse Epiblast Stem cells (EpiSCs) established in the presence of Wnt secretion inhibitor, IWP-2. The cell line was derived from embryos obtained by 129S2/Sv x C57BL/6N cross. Homogeneous cellular state can be maintained in the culture medium containing IWP-2. |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cell Reports 2015;4:744-757. ) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Abe, Kuniya
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| Originator |
Masayo Kondo, Michihiko Sugimoto, Kuniya Abe
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| Year of deposit |
2015
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| Animal |
_mouse
< Mammals
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| Strain name |
129S2/Sv x C57BL/6N hybrid
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
epiblast
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| Classification |
normal
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| Recombinant |
non-recombinant
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| Year of origin |
2017
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|
Lifespan |
infinite
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| Morphology |
other
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| Cellosaurus(Expasy) |
CVCL_C5QL
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM/HamF12 (2.5mM GlutaMAX) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 12ng/ml human basic FGF + 20ng/ml human Activin A + 2 μM IWP-2
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + 0.1% collagenase type IV + 20% KSR + 1mM CaCl2 + PBS(-)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage ratio |
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1 : 6-12 split
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| SC frequency |
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Subculture : once/2 days, Medium Renewal : every day
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10 5 cells/60 mm dish
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| Freeze medium |
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CELLBANKER 2
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
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OK
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| SSLP(mouse) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
4
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| User's Publication |
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19859
Lázaro J, Costanzo M, Sanaki-Matsumiya M, Girardot C, Hayashi M, Hayashi K, Diecke S, Hildebrandt TB, Lazzari G, Wu J, Petkov S, Behr R, Trivedi V, Matsuda M, Ebisuya M.
A stem cell zoo uncovers intracellular scaling of developmental tempo across mammals
Cell Stem Cell
2023
30(7):938-949
PubMed ID: 37343565
DOI: 10.1016/j.stem.2023.05.014
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13934
Ohhata T, Yamazawa K, Miura-Kamio A, Takahashi S, Sakai S, Tamura Y, Uchida C, Kitagawa K, Niida H, Hiratani I, Kobayashi H, Kimura H, Wutz A, Kitagawa M.
Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix
Cell Rep
2021
34(13):108912
PubMed ID: 33789104
DOI: 10.1016/j.celrep.2021.108912
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12112
Matsuda M, Yamanaka Y, Uemura M, Osawa M, Saito MK, Nagahashi A, Nishio M, Guo L, Ikegawa S, Sakurai S, Kihara S, Maurissen TL, Nakamura M, Matsumoto T, Yoshitomi H, Ikeya M, Kawakami N, Yamamoto T, Woltjen K, Ebisuya M, Toguchida J, Alev C.
Recapitulating the human segmentation clock with pluripotent stem cells.
Nature
2020
PubMed ID: 32238941
DOI: 10.1038/s41586-020-2144-9
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11128
Kondo M, Sugimoto M, Abe K.
A Simplified and Efficient Protocol for Derivation and Maintenance of High-Quality Mouse Primed Pluripotent Stem Cells Using Wnt Inhibition.
Curr Protoc Stem Cell Biol
2018
46(1):e60
PubMed ID: 30005143
DOI: 10.1002/cpsc.60
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