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Cell No. : Cell Name
AES0197 : Mili-KO GS  update : 2018/07/31
CommentMouse germline stem (GS) cell lacking Mili gene. Medium and additives(excel:20KB)
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (RNA 2013;19:803-810) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 3) A prior permission of the depositor (S. Kuramochi-Miyagawa) is necessary for any kind of use including academic use and for-profit use. 4) The RECIPIENT, which wants to use the BIOLOGICAL RESOURCE for for-profit research purposes, is requested to enter into a MTA with Osaka University.
approver's address
Osaka University Medical School and Graduate school of Frontier Biosciences
Laboratory of Stem Cell Pathology
2-2 Yamada-oka, Suita, Osaka 565-0871 Japan
Dr.Miyakawa, Satomi
Fax. +81-6-6879-3729
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kuramochi-Miyagawa, Satomi
Originator Kuramochi-Miyagawa, Satomi
Year of deposit 2013
Animal mouse < Mammals
Strain name DBA2/B6
Gender Male
Age at sampling 3 days
Tissue testis
Classification ES
Recombinant recombinant
Exogene lacking Mili gene
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Please check [Comment].
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2.5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10^(5) cells/well/6 well plate
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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4599  Shiromoto Y, Kuramochi-Miyagawa S, Daiba A, Chuma S, Katanaya A, Katsumata A, Nishimura K, Ohtaka M, Nakanishi M, Nakamura T, Yoshinaga K, Asada N, Nakamura S, Yasunaga T, Kojima-Kita K, Itou D, Kimura T, Nakano T.  GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells.  RNA  2013  19(6):803-10  PubMed ID: 23611983

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