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Cell No. : Cell Name
AES0196 : Mvh-Venus GS  update : 2018/07/31
CommentThis GS cell line was derived from testis of new born transgenic mouse, Tg(Mvh-Venus)1Rbrc, carrying Venus gene inserted into the 2nd exon of Mouse Vasa Homolog (Mvh or alternatively called Ddx4) gene. Mvh gene is known to be specifically expressed in male and female germ cells. Medium and additives(excel:20KB)
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genesis 2013;51:498-505.) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Abe, Kuniya
Originator Abe, Kuniya
Year of deposit 2013
Animal mouse < Mammals
Strain name C57BL/6J, DBA2 (mix background)
Gender Male
Age at sampling 1 day
Tissue testis
Classification ES
Recombinant recombinant
Exogene Mouse Vasa homolog (Mvh or Ddx4), Venus
Year of origin 2011
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Please check [Comment].
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.2% gelatin coated dish
Culture information Passage cell No 3 x 10^(5) cells/well/6 well plate
SC frequency Subculture : once/5 days, Medium Renewal : once/2-3days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 5 x 10^(4) cells/well/6 well plate
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
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lot info
Reference information Reference 1
User's Publication 1


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Reference
3610  Shiura H, Ikeda R, Lee J, Sato T, Ogonuki N, Hirose M, Ogura A, Ogawa T, Abe K.  Generation of a novel germline stem cell line expressing a germline-specific reporter in the mouse.  Genesis  2013  51(7):498-505  PubMed ID: 23529902

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User's Publication
11259  Sato T, Ogawa T.  Generating Genetically Engineered Mice Using a Spermatogonial Stem Cell-Mediated Method.  Methods Mol. Biol.  2019    PubMed ID: 30353509



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