Search
CELL BANK Website

Back Back
Cell No. : Cell Name
AES0194 : NAT1(-/-)ES(B12)  update : 2020/03/26
CommentMouse embryonic stem (ES) cell. NAT1 gene is deficient homogeneously.
Comment from the depositor
Terms and conditions1) The RECIPIENT belongs to a not-for-profit academic organization (i.e., a university or another institution of higher education or any nonprofit scientific or educational organization, including government agencies). 2) Attach TERMS and CONDITIONS to MTA (C-0034) .
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor http://www.cira.kyoto-u.ac.jp/
Originator Yamanaka, Shinya
Year of deposit 2013
Animal mouse < Mammals
Strain name 129 SV Jae
Gender Unknown
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene pRTV-NAT1, Neomycin gene
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 15% FBS + 0.1mM NEAA + 0.11mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 1-1.5 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


To topTop
Reference
3671  Yamanaka S, Zhang XY, Maeda M, Miura K, Wang S, Farese RV Jr, Iwao H, Innerarity TL.  Essential role of NAT1/p97/DAP5 in embryonic differentiation and the retinoic acid pathway.  EMBO J  2000  19(20):5533-41  PubMed ID: 11032820

To topTop
User's Publication



Back Back Return Top Page