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Cell No. : Cell Name
AES0187 : B6-6(feeder-free)  update : 2018/07/06
CommentMouse embryonic stem (ES) cell line derived from C57BL/6NCrSlc. Feeder-free cell line (AES0172 B6-6).
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mater Sci Eng C Mater Biol Appl 2014;34:214-designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko
Year of deposit 2012
Original cell AES0172 B6-6
Animal mouse < Mammals
Strain name C57BL/6NCrSlc
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
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lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2μM BIO + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Chromosome mode 40-41,XY,+6,der(6;6)(q10;q10),+8,der(8;8)(q10;q10)[cp9], 38,XY, +9,-10, +11, der(11;11)(q10;q10), -16, -17[1]
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Reference information Reference 1
User's Publication 0

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4557  Nagai A, Hattori T, Hirose M, Ogura A, Nozaki K, Aizawa M, Yamashita K.  Mouse embryonic stem cells cultured under serum- and feeder-free conditions maintain their self-renewal capacity on hydroxyapatite.  Mater Sci Eng C Mater Biol Appl  2014  34:214-20  PubMed ID: 24268252

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