Cell No. : Cell Name
AES0187 : B6-6(feeder-free)
update : 2022/05/27
|
| Comment | Mouse embryonic stem (ES) cell line derived from C57BL/6NCrSlc. Feeder-free cell line (AES0172 B6-6). |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mater Sci Eng C Mater Biol Appl 2014;34:214-designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Ogura, Atsuo
|
| Originator |
Hirose, Michiko
|
| Year of deposit |
2012
|
| Original cell |
AES0172 B6-6
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6NCrSlc
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_RH43
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2μM BIO + 1000U/ml mouse LIF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Coating dish |
|
0.1% gelatin coated dish
|
Culture information
|
Passage cell No |
|
3-5 x 10 5 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/2-3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
free
|
| Freeze medium |
|
CELLBANKER 2
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Chromosome mode |
|
40-41,XY,+6,der(6;6)(q10;q10),+8,der(8;8)(q10;q10)[cp9], 38,XY, +9,-10, +11, der(11;11)(q10;q10), -16, -17[1]
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
1
|
Back
Top
CELL BANK Website