Search
CELL BANK Website

Back Back
Cell No. : Cell Name
AES0187 : B6-6(feeder-free)  update : 2018/07/06
CommentMouse embryonic stem (ES) cell line derived from C57BL/6NCrSlc. Feeder-free cell line (AES0172 B6-6).
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mater Sci Eng C Mater Biol Appl 2014;34:214-designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko
Year of deposit 2012
Original cell AES0172 B6-6
Animal mouse < Mammals
Strain name C57BL/6NCrSlc
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2μM BIO + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Chromosome mode 40-41,XY,+6,der(6;6)(q10;q10),+8,der(8;8)(q10;q10)[cp9], 38,XY, +9,-10, +11, der(11;11)(q10;q10), -16, -17[1]
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


To topTop
Reference
4557  Nagai A, Hattori T, Hirose M, Ogura A, Nozaki K, Aizawa M, Yamashita K.  Mouse embryonic stem cells cultured under serum- and feeder-free conditions maintain their self-renewal capacity on hydroxyapatite.  Mater Sci Eng C Mater Biol Appl  2014  34:214-20  PubMed ID: 24268252

To topTop
User's Publication



Back Back Return Top Page