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Cell No. : Cell Name
AES0184 : EGR-101  update : 2022/05/27
CommentMouse embryonic stem (ES) cell line derived from C57BL/6N.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Transgenic Res 2013;22:195-200) designated by the DEPOSITOR is required. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of from the approver. 3) The RECIPIENT must contact the DRPOSUTOR in the case of application for any patent or commercial use based in the results from the use of the BIOLOGICAL RESOURCE. 4) RECIPIENT whish ants to use the BIOLOGICAL RESOURCE for the purpose other than education or non-for-profit research is requested to enter into a MTA with Osaka University.
approver's address
Osaka university
Genome Information Research Center
3-1 Yamadaoka, Suita-shi, Osaka 565-0871 Japan
Dr.IKAWA, Masahito
Fax. +81-6-6879-8376
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Okabe, Masaru
Originator Fujihara, Yoshitaka
Year of deposit 2012
Animal mouse < Mammals
Strain name C57BL/6N
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_0J01
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (knockout, 2mM L-Glutamine) + 20% FBS + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 1.2-1.5 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3609  Fujihara Y, Kaseda K, Inoue N, Ikawa M, Okabe M.  Production of mouse pups from germline transmission-failed knockout chimeras.  Transgenic Res  2013  22(1):195-200  PubMed ID: 22826106   DOI: 10.1007/s11248-012-9635-x

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