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Cell No. : Cell Name
AES0175 : rdES4  update : 2023/01/06
CommentRabbit embryonic stem cell line. Application consideration
Comment from the depositor
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver . 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 2010;285:31362-31369) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 4) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
Remarks
approver's address
×
English
Address
3311-1 Yakushiji Shimotsuke-shi, Tochigi 329-0498 Japan
Jichi Medical University School of Medicine
Center for Development of Advanced Medical Technology
Animal Resource Laboratory
E-mail. a-honda@jichi.ac.jp
Dr.HONDA Arata
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Honda, Arata
Originator Honda, Arata
Year of deposit 2011
Animal _rabbit < Mammals
Genus Oryctolagus
Species cuniclus
Gender Unknown
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_K093
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 8ng/ml human basic FGF
Antibiotics Free
Passage method Use DMEM/HamF12 (not PBS(-)) for washing. 0.1% Trypsin (DMEM/HamF12)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10 4 cells/60 mm dish
SC frequency Subculture : once/3-4 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 6 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 1-2 x 10 5 cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Chromosome mode 45(15), 46(35) 45〜46,X,-Xor-Y,add(2)(p),+5,add(9)(q),-16,+17,add(17)(p),add(20) (p),+mar1,+mar2[cp20]
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4023  Honda A, Hirose M, Hatori M, Matoba S, Miyoshi H, Inoue K, Ogura A.  Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine.  J Biol Chem  2010  285:31362-31369  PubMed ID: 20670936   DOI: 10.1074/jbc.M110.150540

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