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Cell No. : Cell Name
AES0174 : rdES2-1 update : 2024/11/13 |
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| Comment | Rabbit embryonic stem (ES) cell line.Application consideration | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver . 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 2010;285:31362-31369) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 4) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0032.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Honda, Arata | ||
| Originator | Honda, Arata | |||
| Year of deposit | 2011 | |||
| Animal | _rabbit < Mammals | |||
| Genus | Oryctolagus | |||
| Species | cuniclus | |||
| Gender | Unknown | |||
| Age at sampling | embryo | |||
| Tissue | embryonic stem cells | |||
| Classification | ES | Lifespan | infinite | |
| Morphology | ES-like | |||
| Cellosaurus(Expasy) | CVCL_X723 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM/HamF12 (2mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 8ng/ml human basic FGF | |||
| Antibiotics | Free | Passage method | Use DMEM/HamF12 (not PBS(-)) for washing. 0.1% Trypsin (DMEM/HamF12) | |
| Coating dish | 0.1% gelatin coated dish | Culture information | Passage cell No | 0.5-1 x 10 4 cells/60 mm dish |
| SC frequency | Subculture : once/3-4 days, Medium Renewal : every day | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 6 % | |||
| Feeder cells | MEF | |||
| Feeder treatment | X-rays 5000R (or MMC) | |||
| Feeder seed cells | 1-2 x 10 5 cells/60 mm dish | |||
| Freeze medium | CELLBANKER 2 | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Chromosome mode | 45(50), 45,X,-Xor-Y,add(2)(p),+5,+17add(17)(p),-19,-20,add(21)(q),+mar1, +mar2[20] | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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