Cell No. : Cell Name
AES0173 : Bf1-Venus K/I EB3
update : 2024/11/11
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| Comment | A subline of mouse ES cell line, EB3, in which Venus gene is knocked-in in the Bf1(Foxg1) locus. |
| Comment from the depositor | |
| Terms and conditions | 1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. 2) Distribution of the BIOLOGICAL TRSOURCE to third parties without permission is prohibited. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell 2008;3:519-532) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sasai, Yoshiki
|
| Originator |
Eiraku, Mototsugu
|
| Year of deposit |
2011
|
| Original cell |
EB3
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
| Recombinant |
recombinant
|
| Exogene |
One of Oct3/4 locus is disrupted by Ires-BSD KO vectors (EB3). PGK-neo, Venus
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_0I99
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2000U/ml mouse LIF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Coating dish |
|
0.1% gelatin coated dish
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Culture information
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Passage cell No |
|
0.4-1 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Feeder cells |
|
free
|
| Freeze medium |
|
CELLBANKER 2
|
| Freezing method |
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Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
0
|
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