Search
CELL BANK Website

Back Back
Cell No. : Cell Name
AES0173 : Bf1-Venus K/I EB3  update : 2020/07/03
CommentA subline of mouse ES cell line, EB3, in which Venus gene is knocked-in in the Bf1(Foxg1) locus.
Comment from the depositor
Terms and conditions1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. 2) Distribution of the BIOLOGICAL TRSOURCE to third parties without permission is prohibited. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell 2008;3:519-532) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sasai, Yoshiki
Originator Eiraku, Mototsugu
Year of deposit 2011
Animal mouse < Mammals
Strain name 129/Ola
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene PGK-neo, Venus
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.4-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


To topTop
Reference
4597  Eiraku M, Watanabe K, Matsuo-Takasaki M, Kawada M, Yonemura S, Matsumura M, Wataya T, Nishiyama A, Muguruma K, Sasai Y.  Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals.  Cell Stem Cell  2008  3(5):519-32  PubMed ID: 18983967

To topTop
User's Publication



Back Back Return Top Page