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Cell No. : Cell Name
AES0173 : Bf1-Venus K/I EB3  update : 2024/02/06
CommentA subline of mouse ES cell line, EB3, in which Venus gene is knocked-in in the Bf1(Foxg1) locus.
Comment from the depositor
Terms and conditions1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research. 2) Distribution of the BIOLOGICAL TRSOURCE to third parties without permission is prohibited. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cell Stem Cell 2008;3:519-532) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sasai, Yoshiki
Originator Eiraku, Mototsugu
Year of deposit 2011
Original cell EB3
Animal _mouse < Mammals
Strain name 129/Ola
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene One of Oct3/4 locus is disrupted by Ires-BSD KO vectors (EB3). PGK-neo, Venus
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_0I99
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.4-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 0

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4597  Eiraku M, Watanabe K, Matsuo-Takasaki M, Kawada M, Yonemura S, Matsumura M, Wataya T, Nishiyama A, Muguruma K, Sasai Y.  Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals.  Cell Stem Cell  2008  3(5):519-32  PubMed ID: 18983967   DOI: 10.1016/j.stem.2008.09.002

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