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Cell No. : Cell Name
AES0172 : B6-6  update : 2018/07/06
CommentMouse ES cell line derived from C57BL/6NCrSlc. Contribution to chimeras and germilne transmission have been confirmed.
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko & Ogura, Atsuo
Year of deposit 2010
Animal mouse < Mammals
Strain name C57BL/6NCrSlc
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
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Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10^(5) cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 40-41(20) : 40,XY(16/20) 40,XY,add(5)(E2)(1/20),40,XY,add(14)(D3) (1/20),40,XY,-X,+mar( 1/20),41,XY,+Y (1/20)
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