Cell No. : Cell Name
AES0172 : B6-6
update : 2022/05/27
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| Comment | Mouse ES cell line derived from C57BL/6NCrSlc. Contribution to chimeras and germilne transmission have been confirmed. |
| Comment from the depositor | |
| Terms and conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Ogura, Atsuo
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| Originator |
Hirose, Michiko & Ogura, Atsuo
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| Year of deposit |
2010
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| Animal |
_mouse
< Mammals
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| Strain name |
C57BL/6NCrSlc
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_K094
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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3-5 x 10 5 cells/60 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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2.5-5.0 x 10 5 cells/60 mm dish
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| Freeze medium |
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Medium : 2 x FM1 = 1 : 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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40-41(20) : 40,XY(16/20) 40,XY,add(5)(E2)(1/20),40,XY,add(14)(D3) (1/20),40,XY,-X,+mar( 1/20),41,XY,+Y (1/20)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
1
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