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Cell No. : Cell Name
AES0172 : B6-6  update : 2022/05/27
CommentMouse ES cell line derived from C57BL/6NCrSlc. Contribution to chimeras and germilne transmission have been confirmed.
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko & Ogura, Atsuo
Year of deposit 2010
Animal _mouse < Mammals
Strain name C57BL/6NCrSlc
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_K094
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Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, 1mM Sodium Pyruvate) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 40-41(20) : 40,XY(16/20) 40,XY,add(5)(E2)(1/20),40,XY,add(14)(D3) (1/20),40,XY,-X,+mar( 1/20),41,XY,+Y (1/20)
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Reference information Reference 1
User's Publication 1

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4557  Nagai A, Hattori T, Hirose M, Ogura A, Nozaki K, Aizawa M, Yamashita K.  Mouse embryonic stem cells cultured under serum- and feeder-free conditions maintain their self-renewal capacity on hydroxyapatite.  Mater Sci Eng C Mater Biol Appl  2014  34:214-20  PubMed ID: 24268252   DOI: 10.1016/j.msec.2013.09.012

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User's Publication
15230  Ito S, Hirabayashi K, Moriishi K, Matsui Y, Moriya K, Koike K, Matsuura Y, Shiota K, Yagi S.  Novel sex-dependent differentially methylated regions are demethylated in adult male mouse livers  Biochem Biophys Res Commun  2015  462(4):332-8  PubMed ID: 25960295   DOI: 10.1016/j.bbrc.2015.04.137

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