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Cell No. : Cell Name
AES0166 : CMES40  update : 2022/12/07
CommentCITES. Embryonic stem (ES) cell line derived from common marmoset (Callithrix jacchus). Common marmoset is one of the animals as the object of Convention on International Trade in Endangered Species (CITES). Therefore, according to the CITES the permissions of the regulatory bodies are necessary in both exporting and importing countries.
Comment from the depositor
Terms and conditions1) In case of commercial use, the RECIPIENT should secure a prior consent of the use from the approver . 2) The RECIPIENT must contact the approver in case of research plan including animal production experiments such as chimeric animal production, cloned animal production. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells 2005;23:1304-1313) designated by the DEPOSITOR is required.
approver's address
Central Institute for Experimental Animals

3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, 210-0821 Japan
Fax. +81-44-754-4481
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sasaki, Erika
Originator Sasaki, Erika
Year of deposit 2006
Animal _marmoset < Mammals
Strain name common marmoset
Genus Callithrix
Species jacchus
Gender Female
Age at sampling embryo
Tissue embryo
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_B946
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (knockout, 1mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% Trypsin + 20% KSR + 1mM calcium chloride
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/3-7 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (ICR)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP, AST, PepB
deposit info
lot info
Reference information Reference 1
User's Publication 3

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4029  Sasaki E, Hanazawa K, Kurita R, Akatsuka A, Yoshizaki T, Ishii H, Tanioka Y, Ohnishi Y, Suemizu H, Sugawara A, Tamaoki N, Izawa K, Nakazaki Y, Hamada H, Suemori H, Asano S, Nakatsuji N, Okano H, Tani K.  Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus)  Stem Cells  2005  23:1304-1313  PubMed ID: 16109758   DOI: 10.1634/stemcells.2004-0366

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User's Publication
21081  Shono M, Kishimoto K, Hikabe O, Hayashi M, Semi K, Takashima Y, Sasaki E, Kato K, Hayashi K.  Induction of primordial germ cell-like cells from common marmoset embryonic stem cells by inhibition of WNT and retinoic acid signaling.  Sci Rep  2023  13(1):3186  PubMed ID: 36823310   DOI: 10.1038/s41598-023-29850-z
21156  Kodera T, Takeuchi RF, Takahashi S, Suzuki K, Kassai H, Aiba A, Shiozawa S, Okano H, Osakada F.  Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids.  Biochem Biophys Res Commun  2023  657:119-127  PubMed ID: 37002985   DOI: 10.1016/j.bbrc.2023.03.019
20359  Abe Y, Nakao H, Goto M, Tamano M, Koebis M, Nakao K, Aiba A.  Efficient marmoset genome engineering by autologous embryo transfer and CRISPR/Cas9 technology.  Sci Rep  2021  11(1):20234  PubMed ID: 34642413   DOI: 10.1038/s41598-021-99656-4

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