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Cell No. : Cell Name
AES0166 : CMES40  update : 2018/07/06
CommentEmbryonic stem (ES) cell line derived from common marmoset (Callithrix jacchus). Common marmoset is one of the animals as the object of Convention on International Trade in Endangered Species (CITES). Therefore, according to the CITES the permissions of the regulatory bodies are necessary in both exporting and importing countries.
Comment from the depositor
Terms and conditions1) In case of commercial use, the RECIPIENT should secure a prior consent of the use from the approver . 2) The RECIPIENT must contact the approver in case of research plan including animal production experiments such as chimeric animal production, cloned animal production. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Stem Cells 2005;23:1304-1313) designated by the DEPOSITOR is required.
Remarks
approver's address
×
English
Address
Central Institute for Experimental Animals

3-25-12 Tonomachi, Kawasaki-ku, Kawasaki, 210-0821 Japan
Dr.Sasaki, Erika
Fax. +81-44-754-4481
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sasaki, Erika
Originator Sasaki, Erika
Year of deposit 2006
Animal marmoset < Mammals
Strain name common marmoset
Genus Callithrix
Species jacchus
Gender Male
Age at sampling embryo
Tissue embryo
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (knockout, 1mM L-Glutamine) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 4ng/ml human basic FGF
Antibiotics Free
Passage method 0.25% trypsin + 20% KSR + 1mM calcium chloride
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 3-5 split
SC frequency Subculture : once/3-7 days, Medium Renewal : every day
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (ICR)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10^(5) cells/60 mm dish
Freeze medium CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP, AST, PepB
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
4029  Sasaki E, Hanazawa K, Kurita R, Akatsuka A, Yoshizaki T, Ishii H, Tanioka Y, Ohnishi Y, Suemizu H, Sugawara A, Tamaoki N, Izawa K, Nakazaki Y, Hamada H, Suemori H, Asano S, Nakatsuji N, Okano H, Tani K.  Establishment of novel embryonic stem cell lines derived from the common marmoset (Callithrix jacchus)  Stem Cells  2005  23:1304-1313  PubMed ID: 16109758

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