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Cell No. : Cell Name
AES0165 : Dnmt3b-/- ES (clone 8bb)  update : 2018/07/06
CommentMouse ES cell line lacking Dnmt3b gene.
Comment from the depositor
Terms and conditions1) RECIPIENT must send a copy of the executed MTA(C-0007) for distribution between Recipient and RIKEN BRC to Massachusetts General Hospital's Research and Licensing Office:101 Huntington Ave, 4th Floor, Boston, MA 02215, (617) 954-9500. 2) Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital (MGH) in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic (“name”) of MGH or any of its affiliates, or any of its or their representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. 3) In publishing research results obtained by the use of BIOLOGICAL RESOURCE, a citation of the literature as designated by the DEPOSITOR is required. (Development.122:3195-205.1996 Cell. 99:247-57. 1999) . 4) The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. 5) The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. 6) Recipients shall assume all liability for their use, storage, handing and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposals, of the BIOLOGICAL RESOURCE by Recipient.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Okano, Masaki
Originator En Li & Okano, Masaki
Year of deposit 2010
Animal mouse < Mammals
Strain name 129Sv/Jae
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene IRES-βgeo, PGK-hygromycin
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 1.5-2 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10^(5) cells/60 mm dish
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
4596  Okano M, Bell DW, Haber DA, Li E.  DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development.  Cell  1999  99(3):247-57  PubMed ID: 10555141
4595  Lei H, Oh SP, Okano M, Jüttermann R, Goss KA, Jaenisch R, Li E.  De novo DNA cytosine methyltransferase activities in mouse embryonic stem cells.  Development  1996  122(10):3195-205  PubMed ID: 8898232

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User's Publication
10472  Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.  Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.  Proc. Natl. Acad. Sci. U.S.A.  2017  114:E7479-E7488  PubMed ID: 28827343



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