Cell No. : Cell Name
AES0165 : Dnmt3b-/- ES (clone 8bb)
update : 2024/11/11
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| Comment | Mouse ES cell line lacking Dnmt3b gene. |
| Comment from the depositor | |
| Terms and conditions | 1) RECIPIENT must send a copy of the executed MTA(C-0007) for distribution between Recipient and RIKEN BRC to Massachusetts General Hospital's Research and Licensing Office:101 Huntington Ave, 4th Floor, Boston, MA 02215, (617) 954-9500. 2) Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital (MGH) in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic (“name”) of MGH or any of its affiliates, or any of its or their representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. 3) In publishing research results obtained by the use of BIOLOGICAL RESOURCE, a citation of the literature as designated by the DEPOSITOR is required. (Development.122:3195-205.1996 Cell. 99:247-57. 1999) . 4) The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. 5) The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. 6) Recipients shall assume all liability for their use, storage, handing and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposals, of the BIOLOGICAL RESOURCE by Recipient. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Okano, Masaki
|
| Originator |
En Li & Okano, Masaki
|
| Year of deposit |
2010
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129Sv/Jae
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
| Recombinant |
recombinant
|
| Exogene |
IRES-βgeo, PGK-hygromycin
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_0J17
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
|
Adherent cells
|
| Culture medium |
|
GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
|
0.1% gelatin coated dish
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Culture information
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Passage cell No |
|
1.5-2 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
|
37
℃
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| CO2 concentration |
|
5
%
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| Feeder cells |
|
MEF
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
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| Feeder seed cells |
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3-5 x 10 5 cells/60 mm dish
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
|
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
2
|
| User's Publication |
|
19500
Miura K, Matoba S, Hirose M, Ogura A.
Generation of chimeric mice with spermatozoa fully derived from embryonic stem cells using a triple-target CRISPR method for Nanos3
Biol Reprod
2021
104(1):223-233
PubMed ID: 32965494
DOI: 10.1093/biolre/ioaa176
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10472
Umemura Y, Koike N, Ohashi M, Tsuchiya Y, Meng QJ, Minami Y, Hara M, Hisatomi M, Yagita K.
Involvement of posttranscriptional regulation of Clock in the emergence of circadian clock oscillation during mouse development.
Proc. Natl. Acad. Sci. U.S.A.
2017
114:E7479-E7488
PubMed ID: 28827343
DOI: 10.1073/pnas.1703170114
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