細胞番号 : 細胞名
AES0164 : Dnmt3a-/- ES (clone 6aa)
update : 2025/01/16
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| 細胞特性(Comment:英) | Mouse ES cell line lacking Dnmt3a gene. |
| 細胞特性(日) | マウスES細胞株。Dnmt3a遺伝子を完全欠損している。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) RECIPIENT must send a copy of the executed MTA(C-0007) for distribution between Recipient and RIKEN BRC to Massachusetts General Hospital"s Research and Licensing Office:101 Huntington Ave, 4th Floor, Boston, MA 02215, (617) 954-9500. 2) Any publication or public disclosure of research results obtained by the use of the BIOLOGICAL RESOURCE shall cite Massachusetts General Hospital (MGH) in Boston, MA as the source of the material. However, neither the name, trademark, service mark, logo nor other identifying characteristic (“name”) of MGH or any of its affiliates, or any of its or their representatives or agents, in any advertising, promotional or sales literature, publicity or in any document employed to obtain funds or financing without the prior written approval of the MGH Department of Public Affairs. 3) In publishing research results obtained by the use of BIOLOGICAL RESOURCE, a citation of the literature as designated by the DEPOSITOR is required. (Development.122:3195-205.1996 Cell. 99:247-57. 1999) . 4) The use of the BIOLOGICAL RESOURCE is restricted to academic researchers in non-profit organizations for their internal research and educational purposes. 5) The BIOLOGICAL RESOURCE shall not be used for commercial purposes. Any request for the BIOLOGICAL RESOURCE by a for-profit entity shall be referred to Massachusetts General Hospital through the Research and Licensing Office. 6) Recipients shall assume all liability for their use, storage, handing and disposal of the BIOLOGICAL RESOURCE. The General Hospital Corporation d/b/a Massachusetts General Hospital will not be liable to the Recipients for any loss, claims, matters, damages, costs or liabilities relating to any third party actions, proceedings, investigations, or matters arising from any use, storage, handling, or disposals, of the BIOLOGICAL RESOURCE by Recipient. |
| 使用条件(日) | 英文に準拠、Material Transfer Agreement (C-0007) に、英文の使用条件をご記載またはご添付ください。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0031.pdf
同意書(非営利学術目的)C-0003.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Okano, Masaki
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| 樹立者 |
En Li & Okano, Masaki
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| 寄託日 |
2010
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| 動物種 |
_mouse
< Mammals
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| 動物系統名 |
129Sv/Jae
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| 性別 |
Male
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| 齢 |
embryo
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| 採取組織 |
embryonic stem cells
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| 細胞分類 |
ES
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
IRES-βgeo
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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| Cellosaurus(Expasy) |
CVCL_0J16
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| 培養容器のコーティング |
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0.1% gelatin coated dish
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播種細胞数 |
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1.5-2 x 10 6 cells/100 mm dish
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| 継代・培地交換頻度 |
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Subculture : once/2-3 days
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| フィーダー細胞 |
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MEF
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| フィーダー細胞の処理方法 |
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X-rays 5000R (or MMC)
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| フィーダー細胞の播種数 |
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3-5 x 10 5 cells/60 mm dish
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| マイコプラズマ |
(-)
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| 動物種PCR |
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OK
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| マウス系統検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
2件
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| Reference(日) |
0件
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| 利用者成果(英) |
1件
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| 利用者成果(日) |
0件
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