Cell No. : Cell Name
AES0154 : OLC2-1
update : 2023/03/28
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| Comment | Mouse embryonic stem (ES) cell line derived from 129/Ola. The expression of Oct3/4 can be detected as the expression of ECFP. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 2008;135:909-918) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Niwa, Hitoshi
|
| Originator |
Niwa, Hitoshi
|
| Year of deposit |
2009
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
| Recombinant |
recombinant
|
| Exogene |
Oct3/4-linker-ECFP KI vector
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_U897
|
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deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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0.5-1 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
|
free
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| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
0
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