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Cell No. : Cell Name
AES0153 : OLV2-1  update : 2024/02/06
CommentMouse embryonic stem (ES) cell line derived from 129/Ola. The expression of Oct3/4 can be detected as the expression of Venus.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 2008;135:909-918) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
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Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Animal _mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene Oct3/4-linker-Venus KI vector
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_U896
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 3

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3608  Toyooka Y, Shimosato D, Murakami K, Takahashi K, Niwa H.  Identification and characterization of subpopulations in undifferentiated ES cell culture.  Development  2008  135(5):909-18  PubMed ID: 18263842   DOI: 10.1242/dev.017400

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User's Publication
20547  Ando Y, Okeyo KO, Adachi T.  Pluripotency state of mouse ES cells determines their contribution to self-organized layer formation by mesh closure on microstructured adhesion-limiting substrates.  Biochem Biophys Res Commun  2022  590:97-102  PubMed ID: 34973536   DOI: 10.1016/j.bbrc.2021.12.066
10234  Fukuhara M, Ma Y, Nagasawa K, Toyoshima F.  A G-quadruplex structure at the 5' end of the H19 coding region regulates H19 transcription.  Sci Rep  2017  8:45815  PubMed ID: 28367967   DOI: 10.1038/srep45815
14645  Ikeda M, Toyoshima F.  Dormant Pluripotent Cells Emerge during Neural Differentiation of Em  Mol Cell Biol  2017  37(5):e00417-16  PubMed ID: 27956699   DOI: 10.1128/MCB.00417-16

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