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Cell No. : Cell Name
AES0152 : OCRG9  update : 2018/07/06
CommentMouse embryonic stem cell line derived from 129/Ola.
Comment from the depositor
Terms and conditions1) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Development 2008;135:909-918) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene Rex1-EGFP-IB KI vector, Oct3/4-linker-ECFP KI vectors.
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3608  Toyooka Y, Shimosato D, Murakami K, Takahashi K, Niwa H.  Identification and characterization of subpopulations in undifferentiated ES cell culture.  Development  2008  135(5):909-18  PubMed ID: 18263842

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