細胞番号 : 細胞名
AES0151 : EB5
update : 2020/08/27
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| 細胞特性(Comment:英) | Mouse embryonic stem cell line derived from 129/Ola. Feeder-free cell line. |
| 細胞特性(日) | マウス胚性幹（ES）細胞株。129/Ola由来。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell biol 2002;22:1526-36, Genes Cell 2004;9:471-477) designated by the DEPOSITOR is required. |
| 使用条件(日) | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mol Cell biol 2002;22:1526-36、Genes Cell 2004;9:471-477) を引用すること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類 |
依頼書C-0031.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関及び使用内容に応じて二種類(非営利学術目的・営利目的)あります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は別途ご相談（cellbank.brc@riken.jp）を承ります。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Niwa, Hitoshi
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| 樹立者 |
Niwa, Hitoshi
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| 寄託日 |
2009
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| 動物種 |
mouse
< Mammals
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| 動物系統名 |
129/Ola
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| 性別 |
Male
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| 齢 |
embryo
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| 採取組織 |
embryonic stem cells
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| 細胞分類 |
ES
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| 遺伝子改変 |
recombinant
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| 外来遺伝子 |
One of Oct3/4 locus is disrupted by Ires-BSD KO vectors.
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細胞寿命 |
infinite
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| 細胞形態 |
ES-like
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| 抗生物質 |
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Free
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継代方法 |
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0.25% trypsin + 0.02% EDTA
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| 培養容器のコーティング |
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0.1% gelatin coated dish
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播種細胞数 |
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0.5-1 x 10^(6) cells/100 mm dish
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| 継代・培地交換頻度 |
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Subculture : once/2-3 days
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
|
5
%
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| フィーダー細胞 |
|
free
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| マウス系統検査 |
|
OK
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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40,XY(50)
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
8件
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| Reference(日) |
0件
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| 利用者成果(英) |
9件
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| 利用者成果(日) |
0件
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| Reference(英) |
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4604
Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H5.
Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells.
Development
2018
145(10):dev162404
PubMed ID: 29739838
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4587
Masui S, Ohtsuka S, Yagi R, Takahashi K, Ko MS, Niwa H.
Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.
BMC Dev Biol
2008
8:45
PubMed ID: 18433507
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4588
Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.
Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.
BMC Genomics
2008
9:269
PubMed ID: 18522731
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4586
Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.
Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.
Nat Cell Biol
2007
9(6):625-35
PubMed ID: 17515932
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4584
Nakatake Y, Fukui N, Iwamatsu Y, Masui S, Takahashi K, Yagi R, Yagi K, Miyazaki J, Matoba R, Ko MS, Niwa H.
Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.
Mol Cell Biol
2006
26(20):7772-82
PubMed ID: 16954384
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4585
Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.
Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.
PLoS One
2006
1:e26.
PubMed ID: 17183653
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3605
Ogawa K, Matsui H, Ohtsuka S, Niwa H.
A novel mechanism for regulating clonal propagation of mouse ES cells.
Genes Cells
2004
9(5):471-7
PubMed ID: 15147275
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4579
Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J.
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.
Mol Cell Biol
2002
22(5):1526-36
PubMed ID: 11839818
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| 利用者成果(英) |
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12151
Ichiro Fukunaga, Cheng Chen, Yoko Oe, Keiko Danzaki, Sayaka Ohta, Akito Koike, Ayumi Fujimoto, Katsuhisa Ikeda, Kazusaku Kamiya
TGF-β inhibitor accelerates BMP4-induced cochlear gap junction formation during in vitro differentiation of embryonic stem cells
bioRxiv
2020
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12352
Arno Germond, Yulia Panina, Mikio Shiga, Hirohiko Niioka, Tomonobu M Watanabe
Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy
Anal Chem
2020
92(22):14915-14923
PubMed ID: 33112148
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11427
Nishimura K, Ishiwata H, Sakuragi Y, Hayashi Y, Fukuda A, Hisatake K.
Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.
Sci Rep
2019
PubMed ID: 30741960
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11887
Sano M, Nakasu A, Ohtaka M, Nakanishi M.
A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term Expression of MicroRNAs.
Mol Ther Methods Clin Dev
2019
PubMed ID: 31828179
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11929
Hu Y, Domínguez CM, Bauer J, Weigel S, Schipperges A, Oelschlaeger C, Willenbacher N, Keppler S, Bastmeyer M, Heißler S, Wöll C, Scharnweber T, Rabe KS, Niemeyer CM.
Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings.
Nat Commun
2019
PubMed ID: 31797918
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11245
Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.
Generation of orthotopically functional salivary gland from embryonic stem cells.
Nat Commun
2018
PubMed ID: 30310071
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10121
Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T.
Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons.
Sci Rep
2017
7:42111
PubMed ID: 28176845
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10373
Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.
Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.
Stem Cell Res
2017
23:13-19
PubMed ID: 28666145
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10596
Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.
Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.
Nat Commun
2017
8:1339
PubMed ID: 29109536
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