Cell No. : Cell Name
AES0151 : EB5
update : 2024/11/11
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| Comment | Mouse embryonic stem cell line derived from 129/Ola. Feeder-free cell line. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell biol 2002;22:1526-36, Genes Cell 2004;9:471-477) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Niwa, Hitoshi
|
| Originator |
Niwa, Hitoshi
|
| Year of deposit |
2009
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
| Recombinant |
recombinant
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| Exogene |
One of Oct3/4 locus is disrupted by Ires-BSD KO vectors.
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Lifespan |
infinite
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| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_J648
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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0.5-1 x 10 6 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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free
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Mycoplasma |
(-)
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| SSLP(mouse) |
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OK
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| Isozyme |
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LD, NP
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| Chromosome mode |
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40,XY(50)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
8
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| User's Publication |
12
|
| Reference |
|
4604
Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H5.
Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells.
Development
2018
145(10):dev162404
PubMed ID: 29739838
DOI: 10.1242/dev.162404
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4587
Masui S, Ohtsuka S, Yagi R, Takahashi K, Ko MS, Niwa H.
Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.
BMC Dev Biol
2008
8:45
PubMed ID: 18433507
DOI: 10.1186/1471-213X-8-45
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4588
Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.
Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.
BMC Genomics
2008
9:269
PubMed ID: 18522731
DOI: 10.1186/1471-2164-9-269
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4586
Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.
Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.
Nat Cell Biol
2007
9(6):625-35
PubMed ID: 17515932
DOI: 10.1038/ncb1589
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4584
Nakatake Y, Fukui N, Iwamatsu Y, Masui S, Takahashi K, Yagi R, Yagi K, Miyazaki J, Matoba R, Ko MS, Niwa H.
Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.
Mol Cell Biol
2006
26(20):7772-82
PubMed ID: 16954384
DOI: 10.1128/MCB.00468-06
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4585
Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.
Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.
PLoS One
2006
1:e26.
PubMed ID: 17183653
DOI: 10.1371/journal.pone.0000026
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3605
Ogawa K, Matsui H, Ohtsuka S, Niwa H.
A novel mechanism for regulating clonal propagation of mouse ES cells.
Genes Cells
2004
9(5):471-7
PubMed ID: 15147275
DOI: 10.1111/j.1356-9597.2004.00736.x
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4579
Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J.
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.
Mol Cell Biol
2002
22(5):1526-36
PubMed ID: 11839818
DOI: 10.1128/mcb.22.5.1526-1536.2002
|
| User's Publication |
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21697
Hung SS, Tsai PS, Po CW, Hou PS.
Pax6 isoforms shape eye development: Insights from developmental stages and organoid models.
Differentiation
2024
137:100781
PubMed ID: 38631141
DOI: 10.1016/j.diff.2024.100781
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14067
Fukunaga I, Oe Y, Chen C, Danzaki K, Ohta S, Koike A, Ikeda K, Kamiya K.
Activin/Nodal/TGF-β Pathway Inhibitor Accelerates BMP4-Induced Cochlear Gap Junction Formation During in vitro Differentiation of Embryonic Stem Cells
Front Cell Dev Biol
2021
9:602197
PubMed ID: 33968919
DOI: 10.3389/fcell.2021.602197
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12352
Arno Germond, Yulia Panina, Mikio Shiga, Hirohiko Niioka, Tomonobu M Watanabe
Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy
Anal Chem
2020
92(22):14915-14923
PubMed ID: 33112148
DOI: 10.1021/acs.analchem.0c01800
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11427
Nishimura K, Ishiwata H, Sakuragi Y, Hayashi Y, Fukuda A, Hisatake K.
Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.
Sci Rep
2019
PubMed ID: 30741960
DOI: 10.1038/s41598-018-37779-x
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11887
Sano M, Nakasu A, Ohtaka M, Nakanishi M.
A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term Expression of MicroRNAs.
Mol Ther Methods Clin Dev
2019
PubMed ID: 31828179
DOI: 10.1016/j.omtm.2019.10.012
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11929
Hu Y, Domínguez CM, Bauer J, Weigel S, Schipperges A, Oelschlaeger C, Willenbacher N, Keppler S, Bastmeyer M, Heißler S, Wöll C, Scharnweber T, Rabe KS, Niemeyer CM.
Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings.
Nat Commun
2019
PubMed ID: 31797918
DOI: 10.1038/s41467-019-13381-1
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11245
Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.
Generation of orthotopically functional salivary gland from embryonic stem cells.
Nat Commun
2018
PubMed ID: 30310071
DOI: 10.1038/s41467-018-06469-7
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10121
Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T.
Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons.
Sci Rep
2017
7:42111
PubMed ID: 28176845
DOI: 10.1038/srep42111
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10373
Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.
Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.
Stem Cell Res
2017
23:13-19
PubMed ID: 28666145
DOI: 10.1016/j.scr.2017.06.011
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10596
Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.
Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.
Nat Commun
2017
8:1339
PubMed ID: 29109536
DOI: 10.1038/s41467-017-01105-2
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14645
Ikeda M, Toyoshima F.
Dormant Pluripotent Cells Emerge during Neural Differentiation of Em
Mol Cell Biol
2017
37(5):e00417-16
PubMed ID: 27956699
DOI: 10.1128/MCB.00417-16
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14776
Joshi R, Buchanan JC, Tavana H.
Self-regulatory factors of embryonic stem cells in co-culture with stromal cells enhance neural differentiation
Integr Biol (Camb)
2017
9(5):418-426
PubMed ID: 28406502
DOI: 10.1039/c7ib00038c
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