Back Back
Cell No. : Cell Name
AES0151 : EB5  update : 2023/07/19
CommentMouse embryonic stem cell line derived from 129/Ola. Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell biol 2002;22:1526-36, Genes Cell 2004;9:471-477) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Animal _mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene One of Oct3/4 locus is disrupted by Ires-BSD KO vectors.
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_J648
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 40,XY(50)
deposit info
lot info
Reference information Reference 8
User's Publication 11

To topTop
4604  Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H5.  Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells.  Development  2018  145(10):dev162404  PubMed ID: 29739838   DOI: 10.1242/dev.162404
4587  Masui S, Ohtsuka S, Yagi R, Takahashi K, Ko MS, Niwa H.  Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.  BMC Dev Biol  2008  8:45  PubMed ID: 18433507   DOI: 10.1186/1471-213X-8-45
4588  Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.  Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.  BMC Genomics  2008  9:269  PubMed ID: 18522731   DOI: 10.1186/1471-2164-9-269
4586  Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.  Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.  Nat Cell Biol  2007  9(6):625-35  PubMed ID: 17515932   DOI: 10.1038/ncb1589
4584  Nakatake Y, Fukui N, Iwamatsu Y, Masui S, Takahashi K, Yagi R, Yagi K, Miyazaki J, Matoba R, Ko MS, Niwa H.  Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.  Mol Cell Biol  2006  26(20):7772-82  PubMed ID: 16954384   DOI: 10.1128/MCB.00468-06
4585  Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.  Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.  PLoS One  2006  1:e26.  PubMed ID: 17183653   DOI: 10.1371/journal.pone.0000026
3605  Ogawa K, Matsui H, Ohtsuka S, Niwa H.  A novel mechanism for regulating clonal propagation of mouse ES cells.  Genes Cells  2004  9(5):471-7  PubMed ID: 15147275   DOI: 10.1111/j.1356-9597.2004.00736.x
4579  Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J.  Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.  Mol Cell Biol  2002  22(5):1526-36  PubMed ID: 11839818   DOI: 10.1128/mcb.22.5.1526-1536.2002

To topTop
User's Publication
14067  Fukunaga I, Oe Y, Chen C, Danzaki K, Ohta S, Koike A, Ikeda K, Kamiya K.  Activin/Nodal/TGF-β Pathway Inhibitor Accelerates BMP4-Induced Cochlear Gap Junction Formation During in vitro Differentiation of Embryonic Stem Cells  Front Cell Dev Biol  2021  9:602197  PubMed ID: 33968919   DOI: 10.3389/fcell.2021.602197
12352  Arno Germond, Yulia Panina, Mikio Shiga, Hirohiko Niioka, Tomonobu M Watanabe  Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy  Anal Chem  2020  92(22):14915-14923  PubMed ID: 33112148   DOI: 10.1021/acs.analchem.0c01800
11427  Nishimura K, Ishiwata H, Sakuragi Y, Hayashi Y, Fukuda A, Hisatake K.  Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.  Sci Rep  2019    PubMed ID: 30741960   DOI: 10.1038/s41598-018-37779-x
11887  Sano M, Nakasu A, Ohtaka M, Nakanishi M.  A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term Expression of MicroRNAs.  Mol Ther Methods Clin Dev  2019    PubMed ID: 31828179   DOI: 10.1016/j.omtm.2019.10.012
11929  Hu Y, Domínguez CM, Bauer J, Weigel S, Schipperges A, Oelschlaeger C, Willenbacher N, Keppler S, Bastmeyer M, Heißler S, Wöll C, Scharnweber T, Rabe KS, Niemeyer CM.  Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings.  Nat Commun  2019    PubMed ID: 31797918   DOI: 10.1038/s41467-019-13381-1
11245  Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.  Generation of orthotopically functional salivary gland from embryonic stem cells.  Nat Commun  2018    PubMed ID: 30310071   DOI: 10.1038/s41467-018-06469-7
10121  Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T.  Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons.  Sci Rep  2017  7:42111  PubMed ID: 28176845   DOI: 10.1038/srep42111
10373  Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.  Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.  Stem Cell Res  2017  23:13-19  PubMed ID: 28666145   DOI: 10.1016/j.scr.2017.06.011
10596  Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.  Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.  Nat Commun  2017  8:1339  PubMed ID: 29109536   DOI: 10.1038/s41467-017-01105-2
14645  Ikeda M, Toyoshima F.  Dormant Pluripotent Cells Emerge during Neural Differentiation of Em  Mol Cell Biol  2017  37(5):e00417-16  PubMed ID: 27956699   DOI: 10.1128/MCB.00417-16
14776  Joshi R, Buchanan JC, Tavana H.  Self-regulatory factors of embryonic stem cells in co-culture with stromal cells enhance neural differentiation  Integr Biol (Camb)  2017  9(5):418-426  PubMed ID: 28406502   DOI: 10.1039/c7ib00038c

Back Back Return Top Page