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Cell No. : Cell Name
AES0151 : EB5  update : 2020/08/27
CommentMouse embryonic stem cell line derived from 129/Ola. Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell biol 2002;22:1526-36, Genes Cell 2004;9:471-477) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene One of Oct3/4 locus is disrupted by Ires-BSD KO vectors.
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 40,XY(50)
deposit info
lot info
Reference information Reference 8
User's Publication 8

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4604  Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H5.  Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells.  Development  2018  145(10):dev162404  PubMed ID: 29739838
4587  Masui S, Ohtsuka S, Yagi R, Takahashi K, Ko MS, Niwa H.  Rex1/Zfp42 is dispensable for pluripotency in mouse ES cells.  BMC Dev Biol  2008  8:45  PubMed ID: 18433507
4588  Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.  Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.  BMC Genomics  2008  9:269  PubMed ID: 18522731
4586  Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.  Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.  Nat Cell Biol  2007  9(6):625-35  PubMed ID: 17515932
4584  Nakatake Y, Fukui N, Iwamatsu Y, Masui S, Takahashi K, Yagi R, Yagi K, Miyazaki J, Matoba R, Ko MS, Niwa H.  Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.  Mol Cell Biol  2006  26(20):7772-82  PubMed ID: 16954384
4585  Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.  Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.  PLoS One  2006  1:e26.  PubMed ID: 17183653
3605  Ogawa K, Matsui H, Ohtsuka S, Niwa H.  A novel mechanism for regulating clonal propagation of mouse ES cells.  Genes Cells  2004  9(5):471-7  PubMed ID: 15147275
4579  Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J.  Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.  Mol Cell Biol  2002  22(5):1526-36  PubMed ID: 11839818

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User's Publication
12151  Ichiro Fukunaga, Cheng Chen, Yoko Oe, Keiko Danzaki, Sayaka Ohta, Akito Koike, Ayumi Fujimoto, Katsuhisa Ikeda, Kazusaku Kamiya  TGF-β inhibitor accelerates BMP4-induced cochlear gap junction formation during in vitro differentiation of embryonic stem cells  bioRxiv  2020   
11427  Nishimura K, Ishiwata H, Sakuragi Y, Hayashi Y, Fukuda A, Hisatake K.  Live-cell imaging of subcellular structures for quantitative evaluation of pluripotent stem cells.  Sci Rep  2019    PubMed ID: 30741960
11887  Sano M, Nakasu A, Ohtaka M, Nakanishi M.  A Sendai Virus-Based Cytoplasmic RNA Vector as a Novel Platform for Long-Term Expression of MicroRNAs.  Mol Ther Methods Clin Dev  2019    PubMed ID: 31828179
11929  Hu Y, Domínguez CM, Bauer J, Weigel S, Schipperges A, Oelschlaeger C, Willenbacher N, Keppler S, Bastmeyer M, Heißler S, Wöll C, Scharnweber T, Rabe KS, Niemeyer CM.  Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings.  Nat Commun  2019    PubMed ID: 31797918
11245  Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.  Generation of orthotopically functional salivary gland from embryonic stem cells.  Nat Commun  2018    PubMed ID: 30310071
10121  Izumi Y, Wakita S, Kanbara C, Nakai T, Akaike A, Kume T.  Integrin α5β1 expression on dopaminergic neurons is involved in dopaminergic neurite outgrowth on striatal neurons.  Sci Rep  2017  7:42111  PubMed ID: 28176845
10373  Nishimura K, Ohtaka M, Takada H, Kurisaki A, Tran NVK, Tran YTH, Hisatake K, Sano M, Nakanishi M.  Simple and effective generation of transgene-free induced pluripotent stem cells using an auto-erasable Sendai virus vector responding to microRNA-302.  Stem Cell Res  2017  23:13-19  PubMed ID: 28666145
10596  Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.  Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.  Nat Commun  2017  8:1339  PubMed ID: 29109536

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