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Cell No. : Cell Name
AES0150 : G4-2  update : 2022/05/27
CommentMouse embryonic stem (ES) cell line derived from 29/Ola. Expressing GFP.
Comment from the depositor
Terms and conditions1) The RECIPIENT should read Announcement of GFP bioresources' 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Genet 2000;24:372) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Original cell EB3
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells : EB3
Classification ES
Recombinant recombinant
Exogene 0ne of Oct3/4 locus is disrupted by Ires-BSD KO vectors (EB3). CAG-EGFP-IP
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_E223
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 40(50)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
4578  Niwa H, Miyazaki J, Smith AG.  Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.  Nat Genet  2000  24(4):372-6  PubMed ID: 10742100

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User's Publication
11245  Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.  Generation of orthotopically functional salivary gland from embryonic stem cells.  Nat Commun  2018    PubMed ID: 30310071
15632  Matsuda R, Yoshikawa M, Kimura H, Ouji Y, Nakase H, Nishimura F, Nonaka JI, Toriumi H, Yamada S, Nishiofuku M, Moriya K, Ishizaka S, Nakamura M, Sakaki T.  Cotransplantation of Mouse Embryonic Stem Cells and Bone Marrow Stromal Cells following Spinal Cord Injury Suppresses Tumor Development  Cell Transplant  2009  18(1):39-54  PubMed ID: 28841348



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