Cell No. : Cell Name AES0150 : G4-2  update : 2024/11/11
Comment			Mouse embryonic stem (ES) cell line derived from 29/Ola. Expressing GFP.
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Genet 2000;24:372) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Niwa, Hitoshi
		Originator	Niwa, Hitoshi
		Year of deposit	2009
		Original cell	EB3
		Animal	_mouse < Mammals
		Strain name	129/Ola
		Gender	Male
		Age at sampling	embryo
		Tissue	embryonic stem cells : EB3
		Classification	ES
		Recombinant	recombinant
		Exogene	0ne of Oct3/4 locus is disrupted by Ires-BSD KO vectors (EB3). CAG-EGFP-IP
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_E223
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
		Antibiotics		Free
		Passage method		0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage cell No		0.5-1 x 10 6 cells/100 mm dish
		SC frequency		Subculture : once/2-3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		free
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Mycoplasma	(-)
		SSLP(mouse)		OK
		Isozyme		LD, NP
		Chromosome mode		40(50)
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

4578  Niwa H, Miyazaki J, Smith AG.  Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.  Nat Genet  2000  24(4):372-6  PubMed ID: 10742100   DOI: 10.1038/74199

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User's Publication

11245  Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.  Generation of orthotopically functional salivary gland from embryonic stem cells.  Nat Commun  2018    PubMed ID: 30310071   DOI: 10.1038/s41467-018-06469-7

15632  Matsuda R, Yoshikawa M, Kimura H, Ouji Y, Nakase H, Nishimura F, Nonaka JI, Toriumi H, Yamada S, Nishiofuku M, Moriya K, Ishizaka S, Nakamura M, Sakaki T.  Cotransplantation of Mouse Embryonic Stem Cells and Bone Marrow Stromal Cells following Spinal Cord Injury Suppresses Tumor Development  Cell Transplant  2009  18(1):39-54  PubMed ID: 28841348   DOI: 10.3727/096368909788237122