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Cell No. : Cell Name
AES0150 : G4-2  update : 2018/07/06
CommentMouse embryonic stem (ES) cell line derived from 29/Ola. Expressing GFP.
Comment from the depositor
Terms and conditions1) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.' 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Genet 2000;24:372) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2009
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells : EB3
Classification ES
Recombinant recombinant
Exogene CAG-EGFP-IP
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 40(50)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
4578  Niwa H, Miyazaki J, Smith AG.  Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.  Nat Genet  2000  24(4):372-6  PubMed ID: 10742100

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User's Publication
11245  Tanaka J, Ogawa M, Hojo H, Kawashima Y, Mabuchi Y, Hata K, Nakamura S, Yasuhara R, Takamatsu K, Irié T, Fukada T, Sakai T, Inoue T, Nishimura R, Ohara O, Saito I, Ohba S, Tsuji T, Mishima K.  Generation of orthotopically functional salivary gland from embryonic stem cells.  Nat Commun  2018    PubMed ID: 30310071



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