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Cell No. : Cell Name
AES0149 : aBJ-4  update : 2023/11/21
CommentMouse androgenetic embryonic stem (ES) cell line. C57BL/6Cr x JF1
Comment from the depositor
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genesis 2009;47:155-160) designated by the DEPOSITOR is required.
Remarks
approver's address
×
English
Address
RIKEN, BioResource Research Center

3-1-1 Koyadai,Tsukuba-shi, Ibaraki 305-0074 Japan
Dr.OGURA Atsuo
Fax. +81-29-836-9172
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko
Year of deposit 2008
Animal _mouse < Mammals
Strain name (C57BL/6Cr x JF1)F1
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_0J15
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10 5 cells/60 mm dish
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
SSLP(mouse) OK
Chromosome mode 40,XX(20)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3607  Miki H, Hirose M, Ogonuki N, Inoue K, Kezuka F, Honda A, Mekada K, Hanaki K, Iwafune H, Yoshiki A, Ishino F, Ogura A.  Efficient production of androgenetic embryos by round spermatid injection.  Genesis  2009  47(3):155-60  PubMed ID: 19241381   DOI: 10.1002/dvg.20451

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