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Cell No. : Cell Name
AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)  update : 2018/07/06
CommentMouse ES cell line lacking all of Dnmt1, Dnmt3a and Dnmt3b. Derived from 129Sv/Jae.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2006;11:805-814) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Okano, Masaki
Originator Tsumura, Akiko & Okano, Masaki
Year of deposit 2008
Animal mouse < Mammals
Strain name 129Sv/Jae
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene LacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 15% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 4-10 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
4594  Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, Shimotohno K, Ishikawa F, Li E, Ueda HR, Nakayama J, Okano M.  10.1111/j.1365-2443.2006.00984.x  Genes Cells  2006  11(7):805-14.  PubMed ID: 16824199

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User's Publication
11471  Seah MKY, Wang Y, Goy PA, Loh HM, Peh WJ, Low DHP, Han BY, Wong E, Leong EL, Wolf G, Mzoughi S, Wollmann H, Macfarlan TS, Guccione E, Messerschmidt DM.  The KRAB-zinc-finger protein ZFP708 mediates epigenetic repression at RMER19B retrotransposons.  Development  2019    PubMed ID: 30846446
11236  Chatterjee B, Lin MH, Chen CC, Peng KL, Wu MS, Tseng MC, Chen YJ, Shen CJ.  DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.  Biochim Biophys Acta Gene Regul Mech  2018    PubMed ID: 30300721
7029  Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, Wakayama T, Yamamoto T, Yamada Y.  Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation.  Nature  2017  548:224-227  PubMed ID: 28746308
7032  Yamazaki T, Hatano Y, Handa T, Kato S, Hoida K, Yamamura R, Fukuyama T, Uematsu T, Kobayashi N, Kimura H, Yamagata K.  Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase.  PLoS ONE  2017  12:e0177764  PubMed ID: 28542388
10326  Miyamoto K, Uechi A, Saito K.  The zinc finger domain of RING finger protein 141 reveals a unique RING fold.  Protein Sci.  2017  26:1681-1686  PubMed ID: 28547869



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