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Cell No. : Cell Name
AES0146 : Dnmt1-/-Dnmt3a-/-Dnmt3b-/- ES (clone19)  update : 2023/07/18
CommentMouse ES cell line lacking all of Dnmt1, Dnmt3a and Dnmt3b. Derived from 129Sv/Jae.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2006;11:805-814) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Okano, Masaki
Originator Tsumura, Akiko & Okano, Masaki
Year of deposit 2008
Animal _mouse < Mammals
Strain name 129Sv/Jae
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene LacZ, Neomycin resistance gene, Hygromycin resistance gene, Puromycin resistance gene, IRES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_1T48
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 15% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 4-10 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 7

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4594  Tsumura A, Hayakawa T, Kumaki Y, Takebayashi S, Sakaue M, Matsuoka C, Shimotohno K, Ishikawa F, Li E, Ueda HR, Nakayama J, Okano M.  Maintenance of self-renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt1, Dnmt3a and Dnmt3b  Genes Cells  2006  11(7):805-14.  PubMed ID: 16824199   DOI: 10.1111/j.1365-2443.2006.00984.x

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User's Publication
20967  Mukamel Z, Lifshitz A, Mittnenzweig M, Chomsky E, Schwartzman O, Ben-Kiki O, Zerbib M, Tanay A.  DNA methyltransferases 3A and 3B target specific sequences during mouse gastrulation.  Nat Struct Mol Biol  2022    PubMed ID: 36510023   DOI: 10.1038/s41594-022-00885-6
11471  Seah MKY, Wang Y, Goy PA, Loh HM, Peh WJ, Low DHP, Han BY, Wong E, Leong EL, Wolf G, Mzoughi S, Wollmann H, Macfarlan TS, Guccione E, Messerschmidt DM.  The KRAB-zinc-finger protein ZFP708 mediates epigenetic repression at RMER19B retrotransposons.  Development  2019    PubMed ID: 30846446   DOI: 10.1242/dev.170266
11236  Chatterjee B, Lin MH, Chen CC, Peng KL, Wu MS, Tseng MC, Chen YJ, Shen CJ.  DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.  Biochim Biophys Acta Gene Regul Mech  2018    PubMed ID: 30300721   DOI: 10.1016/j.bbagrm.2018.09.009
7029  Yagi M, Kishigami S, Tanaka A, Semi K, Mizutani E, Wakayama S, Wakayama T, Yamamoto T, Yamada Y.  Derivation of ground-state female ES cells maintaining gamete-derived DNA methylation.  Nature  2017  548:224-227  PubMed ID: 28746308   DOI: 10.1038/nature23286
7032  Yamazaki T, Hatano Y, Handa T, Kato S, Hoida K, Yamamura R, Fukuyama T, Uematsu T, Kobayashi N, Kimura H, Yamagata K.  Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase.  PLoS ONE  2017  12:e0177764  PubMed ID: 28542388   DOI: 10.1371/journal.pone.0177764
10326  Miyamoto K, Uechi A, Saito K.  The zinc finger domain of RING finger protein 141 reveals a unique RING fold.  Protein Sci.  2017  26:1681-1686  PubMed ID: 28547869   DOI: 10.1002/pro.3201
14051  Saito Y, Kunitomi A, Seki T, Tohyama S, Kusumoto D, Takei M, Kashimura S, Hashimoto H, Yozu G, Motoda C, Shimojima M, Egashira T, Oda M, Fukuda K, Yuasa S.  Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells  FEBS Lett   2017  591(18):2879-2889  PubMed ID: 28805244   DOI: 10.1002/1873-3468.12791

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