Cell No. : Cell Name
AES0145 : Rx-GFP K/I EB5
update : 2025/01/16
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| Comment | A subline of mouse embryonic stem cell line, EB5 (129/Ola), in which GFP gene is knocked-in under Rx promoter. |
| Comment from the depositor | |
| Terms and conditions | 1) This cell line is available for academic use, but not for commercial use. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 2008;105:11796-11801) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sasai, Yoshiki
|
| Originator |
Sasai, Yoshiki
|
| Year of deposit |
2008
|
| Original cell |
EB5
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
| Recombinant |
recombinant
|
| Exogene |
One of Oct3/4 locus is disrupted by Ires-BSD KO vectors (EB5). Rax-GFP
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|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_J650
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
|
GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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0.4-1 x 10 6 cells/100 mm dish
|
| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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free
|
| Freeze medium |
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Medium : 2 x FM1 = 1 : 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Mycoplasma |
(-)
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|
| SSLP(mouse) |
|
OK
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| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
7
|
| Reference |
|
5347
Okuda S, Takata N, Hasegawa Y, Kawada M, Inoue Y, Adachi T, Sasai Y, Eiraku M1,.
Strain-triggered mechanical feedback in self-organizing optic-cup morphogenesis.
Sci Adv
2018
4(11):eaau1354
PubMed ID: 30474058
DOI: 10.1126/sciadv.aau1354
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15637
Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.
Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism
Nat Commun
2017
8(1):1339
PubMed ID: 29109536
DOI: 10.1038/s41467-017-01105-2
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15727
Mototsugu Eiraku, Yoshiki Sasai
Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues
Nat Protoc
2011
7(1):69-79
PubMed ID: 22179593
DOI: 10.1038/nprot.2011.429
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3606
Wataya T, Ando S, Muguruma K, Ikeda H, Watanabe K, Eiraku M, Kawada M, Takahashi J, Hashimoto N, Sasai Y.
Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation.
Proc Natl Acad Sci U S A.
2008
105(33):11796-801
PubMed ID: 18697938
DOI: 10.1073/pnas.0803078105
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| User's Publication |
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19628
Ong ALC, Kokaji T, Kishi A, Takihara Y, Shinozuka T, Shimamoto R, Isotani A, Shirai M, Sasai N.
Acquisition of neural fate by combination of BMP blockade and chromatin modification
iScience
2023
26(10):107887
PubMed ID: 37771660
DOI: 10.1016/j.isci.2023.107887
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19792
Owen LJ, Rainger J, Bengani H, Kilanowski F, FitzPatrick DR, Papanastasiou AS.
Characterization of an eye field-like state during optic vesicle organoid development
Development
2023
150(15):dev201432
PubMed ID: 37306293
DOI: 10.1242/dev.201432
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21319
Takata N, Miska JM, Morgan MA, Patel P, Billingham LK, Joshi N, Schipma MJ, Dumar ZJ, Joshi NR, Misharin AV, Embry RB, Fiore L, Gao P, Diebold LP, McElroy GS, Shilatifard A, Chandel NS, Oliver G.
Lactate-dependent transcriptional regulation controls mammalian eye morphogenesis.
Nat Commun
2023
14(1):4129
PubMed ID: 37452018
DOI: 10.1038/s41467-023-39672-2
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12002
Li Q, Mao F, Zhou B, Huang Y, Zou Z, denDekker AD, Xu J, Hou S, Liu J, Dou Y, Rao RC.
p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells.
Cell Rep
2020
PubMed ID: 31940490
DOI: 10.1016/j.celrep.2019.12.039
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21863
Kegeles E, Naumov A, Karpulevich EA, Volchkov P, Baranov P.
Convolutional Neural Networks Can Predict Retinal Differentiation in Retinal Organoids
Front Cell Neurosci
2020
14:171
PubMed ID: 32719585
DOI: 10.3389/fncel.2020.00171
|
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10298
Aldiri I, Xu B, Wang L, Chen X, Hiler D, Griffiths L, Valentine M, Shirinifard A, Thiagarajan S, Sablauer A, Barabas ME, Zhang J, Johnson D, Frase S, Zhou X, Easton J, Zhang J, Mardis ER, Wilson RK, Downing JR, Dyer MA, .
The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis.
Neuron
2017
94:550-568.e10
PubMed ID: 28472656
DOI: 10.1016/j.neuron.2017.04.022
|
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12925
Hiler DJ, Barabas ME, Griffiths LM, Dyer MA.
Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures
Nat Protoc
2016
11(10):1955-1976
PubMed ID: 27658012
DOI: 10.1038/nprot.2016.109
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