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Cell No. : Cell Name
AES0145 : Rx-GFP K/I EB5  update : 2018/07/06
CommentA subline of mouse embryonic stem cell line, EB5 (129/Ola), in which GFP gene is knocked-in under Rx promoter.
Comment from the depositor
Terms and conditions1) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences. 2) This cell line is available for academic use, but not for commercial use. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 2008;105:11796-11801) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Sasai, Yoshiki
Originator Sasai, Yoshiki
Year of deposit 2008
Animal mouse < Mammals
Strain name 129/Ola
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene Rax-GFP
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.4-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 4

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3606  Wataya T, Ando S, Muguruma K, Ikeda H, Watanabe K, Eiraku M, Kawada M, Takahashi J, Hashimoto N, Sasai Y.  Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation.  Proc Natl Acad Sci U S A.  2008  105(33):11796-801  PubMed ID: 18697938

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User's Publication
12002  Li Q, Mao F, Zhou B, Huang Y, Zou Z, denDekker AD, Xu J, Hou S, Liu J, Dou Y, Rao RC.  p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells.  Cell Rep  2020    PubMed ID: 31940490
5347  Okuda S, Takata N, Hasegawa Y, Kawada M, Inoue Y, Adachi T, Sasai Y, Eiraku M1,.  Strain-triggered mechanical feedback in self-organizing optic-cup morphogenesis.  Sci Adv  2018  4(11):eaau1354  PubMed ID: 30474058
10298  Aldiri I, Xu B, Wang L, Chen X, Hiler D, Griffiths L, Valentine M, Shirinifard A, Thiagarajan S, Sablauer A, Barabas ME, Zhang J, Johnson D, Frase S, Zhou X, Easton J, Zhang J, Mardis ER, Wilson RK, Downing JR, Dyer MA, .  The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis.  Neuron  2017  94:550-568.e10  PubMed ID: 28472656
10596  Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.  Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism.  Nat Commun  2017  8:1339  PubMed ID: 29109536

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