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Cell No. : Cell Name
AES0145 : Rx-GFP K/I EB5  update : 2023/07/19
CommentA subline of mouse embryonic stem cell line, EB5 (129/Ola), in which GFP gene is knocked-in under Rx promoter.
Comment from the depositor
Terms and conditions1) This cell line is available for academic use, but not for commercial use. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 2008;105:11796-11801) designated by the DEPOSITOR is required. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sasai, Yoshiki
Originator Sasai, Yoshiki
Year of deposit 2008
Original cell EB5
Animal _mouse < Mammals
Strain name 129/Ola
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene One of Oct3/4 locus is disrupted by Ires-BSD KO vectors (EB5). Rax-GFP
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_J650
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 1% FBS + 10% KSR + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 2000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.4-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 6

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5347  Okuda S, Takata N, Hasegawa Y, Kawada M, Inoue Y, Adachi T, Sasai Y, Eiraku M1,.  Strain-triggered mechanical feedback in self-organizing optic-cup morphogenesis.  Sci Adv  2018  4(11):eaau1354  PubMed ID: 30474058   DOI: 10.1126/sciadv.aau1354
15637  Takata N, Sakakura E, Eiraku M, Kasukawa T, Sasai Y.  Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism  Nat Commun  2017  8(1):1339  PubMed ID: 29109536   DOI: 10.1038/s41467-017-01105-2
15727  Mototsugu Eiraku, Yoshiki Sasai  Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues  Nat Protoc  2011  7(1):69-79  PubMed ID: 22179593   DOI: 10.1038/nprot.2011.429
3606  Wataya T, Ando S, Muguruma K, Ikeda H, Watanabe K, Eiraku M, Kawada M, Takahashi J, Hashimoto N, Sasai Y.  Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation.  Proc Natl Acad Sci U S A.  2008  105(33):11796-801  PubMed ID: 18697938   DOI: 10.1073/pnas.0803078105

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User's Publication
19628  Ong ALC, Kokaji T, Kishi A, Takihara Y, Shinozuka T, Shimamoto R, Isotani A, Shirai M, Sasai N.  Acquisition of neural fate by combination of BMP blockade and chromatin modification  iScience  2023  26(10):107887  PubMed ID: 37771660   DOI: 10.1016/j.isci.2023.107887
19792  Owen LJ, Rainger J, Bengani H, Kilanowski F, FitzPatrick DR, Papanastasiou AS.  Characterization of an eye field-like state during optic vesicle organoid development  Development  2023  150(15):dev201432  PubMed ID: 37306293   DOI: 10.1242/dev.201432
21319  Takata N, Miska JM, Morgan MA, Patel P, Billingham LK, Joshi N, Schipma MJ, Dumar ZJ, Joshi NR, Misharin AV, Embry RB, Fiore L, Gao P, Diebold LP, McElroy GS, Shilatifard A, Chandel NS, Oliver G.  Lactate-dependent transcriptional regulation controls mammalian eye morphogenesis.  Nat Commun  2023  14(1):4129  PubMed ID: 37452018   DOI: 10.1038/s41467-023-39672-2
12002  Li Q, Mao F, Zhou B, Huang Y, Zou Z, denDekker AD, Xu J, Hou S, Liu J, Dou Y, Rao RC.  p53 Integrates Temporal WDR5 Inputs during Neuroectoderm and Mesoderm Differentiation of Mouse Embryonic Stem Cells.  Cell Rep  2020    PubMed ID: 31940490   DOI: 10.1016/j.celrep.2019.12.039
10298  Aldiri I, Xu B, Wang L, Chen X, Hiler D, Griffiths L, Valentine M, Shirinifard A, Thiagarajan S, Sablauer A, Barabas ME, Zhang J, Johnson D, Frase S, Zhou X, Easton J, Zhang J, Mardis ER, Wilson RK, Downing JR, Dyer MA, .  The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis.  Neuron  2017  94:550-568.e10  PubMed ID: 28472656   DOI: 10.1016/j.neuron.2017.04.022
12925  Hiler DJ, Barabas ME, Griffiths LM, Dyer MA.  Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures  Nat Protoc  2016  11(10):1955-1976  PubMed ID: 27658012   DOI: 10.1038/nprot.2016.109

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