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Cell No. : Cell Name
AES0144 : B6N-22^(UTR)  update : 2022/05/27
CommentMouse ES cell line derived from C57BL/6NCrlCrlj.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) The users should sign an MTA with University of Tsukuba.
Remarks
approver's address
×
English
Address
University of tsukuba

1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575 Japan
Dr.SUGIYAMA Fumihiro
Fax. +81-29-853-3380
Order Form Order Form(C-0032.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugiyama, Fumihiro
Originator Sugiyama, Fumihiro
Year of deposit 2008
Animal _mouse < Mammals
Strain name C57BL/6NCrlCrlj
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_0J04
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (BALB/c)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10 5 cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Chromosome mode 40,XY(50)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3670  Tanimoto Y, Iijima S, Hasegawa Y, Suzuki Y, Daitoku Y, Mizuno S, Ishige T, Kudo T, Takahashi S, Kunita S, Sugiyama F, Yagami K.  Embryonic stem cells derived from C57BL/6J and C57BL/6N mice.  Comp Med  2008  58(4):347-52  PubMed ID: 18724776  

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