Cell No. : Cell Name AES0143 : B6J-23^(UTR)  update : 2023/05/08
Comment			Mouse ES cell line derived from C57BL/6J.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Sugiyama, Fumihiro
		Originator	Sugiyama, Fumihiro
		Year of deposit	2008
		Animal	_mouse < Mammals
		Strain name	C57BL/6J
		Gender	Male
		Age at sampling	embryo
		Tissue	embryonic stem cells
		Classification	ES
		Lifespan	infinite
		Morphology	ES-like
		Cellosaurus(Expasy)	CVCL_0J02
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (high glucose) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
		Antibiotics		Free
		Passage method		0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
		Coating dish		0.1% gelatin coated dish
	Culture information	Passage cell No		3-5 x 10 5 cells/60 mm dish
		SC frequency		Subculture : once/2-3 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Feeder cells		MEF (BALB/c)
		Feeder treatment		X-rays 5000R (or MMC)
		Feeder seed cells		3-5 x 10 5 cells/60 mm dish
		Freeze medium		Medium : 2 x FM1 = 1 : 1
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Isozyme		LD, NP
		Chromosome mode		40,XY(50)
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	2

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Reference

3670  Tanimoto Y, Iijima S, Hasegawa Y, Suzuki Y, Daitoku Y, Mizuno S, Ishige T, Kudo T, Takahashi S, Kunita S, Sugiyama F, Yagami K.  Embryonic stem cells derived from C57BL/6J and C57BL/6N mice.  Comp Med  2008  58(4):347-52  PubMed ID: 18724776

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User's Publication

11633  Seno A, Murakami C, El-Aarag B, Iwasaki Y, Ohara T, Seno M.  Cancer stem cell induction from mouse embryonic stem cells.  Oncol Lett  2019    PubMed ID: 31452753   DOI: 10.3892/ol.2019.10614

7072  Kunitomi A, Yuasa S, Sugiyama F, Saito Y, Seki T, Kusumoto D, Kashimura S, Takei M, Tohyama S, Hashimoto H, Egashira T, Tanimoto Y, Mizuno S, Tanaka S, Okuno H, Yamazawa K, Watanabe H, Oda M, Kaneda R, Matsuzaki Y, Nagai T, Okano H, Yagami K, Tanaka M, Fukuda K.  H1foo Has a Pivotal Role in Qualifying Induced Pluripotent Stem Cells.  Stem Cell Reports  2016  6:825-33  PubMed ID: 27237376   DOI: 10.1016/j.stemcr.2016.04.015