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Cell No. : Cell Name
AES0140 : B6J-S1^(UTR)  update : 2022/12/20
CommentMouse ES cell line derived from C57BL/6J.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sugiyama, Fumihiro
Originator Sugiyama, Fumihiro
Year of deposit 2008
Animal _mouse < Mammals
Strain name C57BL/6J
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_0J03
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 3-5 x 10 5 cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF (BALB/c)
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 3-5 x 10 5 cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 40,XY(50)
deposit info
lot info
Reference information Reference 1
User's Publication 3

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3670  Tanimoto Y, Iijima S, Hasegawa Y, Suzuki Y, Daitoku Y, Mizuno S, Ishige T, Kudo T, Takahashi S, Kunita S, Sugiyama F, Yagami K.  Embryonic stem cells derived from C57BL/6J and C57BL/6N mice.  Comp Med  2008  58(4):347-52  PubMed ID: 18724776  

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User's Publication
13161  Hasan ASH, Dinh TTH, LE HT, Mizuno-Iijima S, Daitoku Y, Ishida M, Tanimoto Y, Kato K, Yoshiki A, Murata K, Mizuno S, Sugiyama F.  Characterization of a bicistronic knock-in reporter mouse model for investigating the role of CABLES2 in vivo.  Exp Anim  2021  70(1):22-30  PubMed ID: 32779618   DOI: 10.1538/expanim.20-0063
20173  Omori H, Otsu M, Nogami H, Shibata M.  Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.  PLoS One  2021  16(4):e0249954  PubMed ID: 33852623   DOI: 10.1371/journal.pone.0249954
11213  Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Takato T, Toyoda A, Okano H, Hishikawa K.  Control of directionality of chromatin folding for the inter- and intra-domain contacts at the Tfap2c-Bmp7 locus.  Epigenetics Chromatin  2018    PubMed ID: 30213272   DOI: 10.1186/s13072-018-0221-1

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