Cell No. : Cell Name
AES0140 : B6J-S1^(UTR)
update : 2022/12/20
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| Comment | Mouse ES cell line derived from C57BL/6J. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sugiyama, Fumihiro
|
| Originator |
Sugiyama, Fumihiro
|
| Year of deposit |
2008
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL/6J
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
|
| Cellosaurus(Expasy) |
CVCL_0J03
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 20% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
|
| Antibiotics |
|
Free
|
|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
|
3-5 x 10 5 cells/60 mm dish
|
| SC frequency |
|
Subculture : once/2-3 days
|
| Temperature |
|
37
℃
|
| CO2 concentration |
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5
%
|
| Feeder cells |
|
MEF (BALB/c)
|
| Feeder treatment |
|
X-rays 5000R (or MMC)
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| Feeder seed cells |
|
3-5 x 10 5 cells/60 mm dish
|
| Freeze medium |
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Medium : 2 x FM1 = 1 : 1
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| Freezing method |
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Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
40,XY(50)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
3
|
| User's Publication |
|
13161
Hasan ASH, Dinh TTH, LE HT, Mizuno-Iijima S, Daitoku Y, Ishida M, Tanimoto Y, Kato K, Yoshiki A, Murata K, Mizuno S, Sugiyama F.
Characterization of a bicistronic knock-in reporter mouse model for investigating the role of CABLES2 in vivo.
Exp Anim
2021
70(1):22-30
PubMed ID: 32779618
DOI: 10.1538/expanim.20-0063
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20173
Omori H, Otsu M, Nogami H, Shibata M.
Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.
PLoS One
2021
16(4):e0249954
PubMed ID: 33852623
DOI: 10.1371/journal.pone.0249954
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11213
Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Takato T, Toyoda A, Okano H, Hishikawa K.
Control of directionality of chromatin folding for the inter- and intra-domain contacts at the Tfap2c-Bmp7 locus.
Epigenetics Chromatin
2018
PubMed ID: 30213272
DOI: 10.1186/s13072-018-0221-1
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