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Cell No. : Cell Name
AES0139 : EB3  update : 2018/07/06
CommentMouse embryonic stem cell line derived from 129/Ola strain. Oct3/4 gene was replaced with 'ires-blasticidin S registant gene' in one allele. Feeder-free cell line. Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell biol 2002;22:1526-36, Genes Cell 2004;9:471-477) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2008
Another name RCB2717
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene One of Oct3/4 locus is disrupted by Ires-BSD KO vectors.
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 6

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4583  Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H.  An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.  Nucleic Acids Res  2005  33(4):e43  PubMed ID: 15741176
3605  Ogawa K, Matsui H, Ohtsuka S, Niwa H.  A novel mechanism for regulating clonal propagation of mouse ES cells.  Genes Cells  2004  9(5):471-7  PubMed ID: 15147275
4581  Fujikura J, Yamato E, Yonemura S, Hosoda K, Masui S, Nakao K, Miyazaki Ji J, Niwa H.  Differentiation of embryonic stem cells is induced by GATA factors.  Genes Dev  2002  16(7):784-9  PubMed ID: 11937486

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User's Publication
11834  Kim SM, Wada KI, Ueki M, Hosokawa K, Maeda M, Sakai Y, Ito Y.  Cytoplasmic fusion between an enlarged embryonic stem cell and a somatic cell using a microtunnel device.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31594640
10836  Umegaki Y, Martinéz Brotons A, Nakanishi Y, Luo Z, Zhang H, Bonni A, Ikeuchi Y.  Palladin is a Neuron-Specific Translational Target of mTOR Signaling that Regulates Axon Morphogenesis.  J. Neurosci.  2018  38(21):4985-4995  PubMed ID: 29712777
10245  Kasai K, Kimura Y, Miyata S.  Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates.  Mater Sci Eng C Mater Biol Appl  2017  78:354-361  PubMed ID: 28575995
7322  Loh XJ, Wu YL.  Cationic star copolymers based on β-cyclodextrins for efficient gene delivery to mouse embryonic stem cell colonies.  Chem. Commun. (Camb.)  2015  51:10815-8  PubMed ID: 26040469
6208  Ogawa Y, Tanaka M, Tanabe M, Suzuki T, Togawa T, Fukushige T, Kanekura T, Sakuraba H, Oishi K.  Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease.  PLoS One  2013  8(1):e55856  PubMed ID: 23383290
11715  Michihiko Sugimoto , Masayo Kondo, Michiko Hirose, Misao Suzuki, Kazuyuki Mekada, Takaya Abe, Hiroshi Kiyonari, Atsuo Ogura, Nobuo Takagi, Karen Artzt, Kuniya Abe  Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions  Cell Rep  2012  2(5):1363-74  PubMed ID: 23142660

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