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Cell No. : Cell Name
AES0139 : EB3  update : 2022/05/27
CommentMouse embryonic stem cell line derived from 129/Ola strain. Oct3/4 gene was replaced with 'ires-blasticidin S registant gene' in one allele. Feeder-free cell line. Feeder-free cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Cell biol 2002;22:1526-36, Genes Cell 2004;9:471-477) designated by the DEPOSITOR is required.
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Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2008
Another name RCB2717
Original cell E14TG2a
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene One of Oct3/4 locus is disrupted by Ires-BSD KO vectors.
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_J647
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
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lot info
Reference information Reference 3
User's Publication 11

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4583  Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H.  An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.  Nucleic Acids Res  2005  33(4):e43  PubMed ID: 15741176
3605  Ogawa K, Matsui H, Ohtsuka S, Niwa H.  A novel mechanism for regulating clonal propagation of mouse ES cells.  Genes Cells  2004  9(5):471-7  PubMed ID: 15147275
4581  Fujikura J, Yamato E, Yonemura S, Hosoda K, Masui S, Nakao K, Miyazaki Ji J, Niwa H.  Differentiation of embryonic stem cells is induced by GATA factors.  Genes Dev  2002  16(7):784-9  PubMed ID: 11937486

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User's Publication
20705  Kiryo T, Takahashi Y, Miyata S.  Purification of pluripotent embryonic stem cells using dielectrophoresis and a flow control system.  Eng Life Sci  2022  22(5):417-426  PubMed ID: 35573134
20720  Sugimoto M, Tada Y, Shichino S, Koyamatsu S, Tsumaki N, Abe K.  Universal Surface Biotinylation (USB): A simple, versatile and cost-effective sample multiplexing method for single-cell RNA-seq analysis.  DNA Res  2022    PubMed ID: 35652718
14075  Suzuki H, Kasai K, Kimura Y, Miyata S.  UV/ozone surface modification combined with atmospheric pressure plasma irradiation for cell culture plastics to improve pluripotent stem cell culture  Mater Sci Eng C Mater Biol Appl  2021  123:112012  PubMed ID: 33812631
13618  Takahashi Y, Miyata S.  Continuous ES/Feeder Cell-Sorting Device Using Dielectrophoresis and Controlled Fluid Flow  Micromachines (Basel)  2020  11(8):734  PubMed ID: 32751153
11834  Kim SM, Wada KI, Ueki M, Hosokawa K, Maeda M, Sakai Y, Ito Y.  Cytoplasmic fusion between an enlarged embryonic stem cell and a somatic cell using a microtunnel device.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31594640
10836  Umegaki Y, Martinéz Brotons A, Nakanishi Y, Luo Z, Zhang H, Bonni A, Ikeuchi Y.  Palladin is a Neuron-Specific Translational Target of mTOR Signaling that Regulates Axon Morphogenesis.  J. Neurosci.  2018  38(21):4985-4995  PubMed ID: 29712777
10245  Kasai K, Kimura Y, Miyata S.  Improvement of adhesion and proliferation of mouse embryonic stem cells cultured on ozone/UV surface-modified substrates.  Mater Sci Eng C Mater Biol Appl  2017  78:354-361  PubMed ID: 28575995
7322  Loh XJ, Wu YL.  Cationic star copolymers based on β-cyclodextrins for efficient gene delivery to mouse embryonic stem cell colonies.  Chem. Commun. (Camb.)  2015  51:10815-8  PubMed ID: 26040469
15234  Arahara K, Matsumoto T, Morimatsu F, Arai K.  Fibronectin modified expression of Sonic hedgehog in ATRA-mediated neuronal differentiation  J Vet Med Sci  2015  77(11):1503-6  PubMed ID: 26051001
6208  Ogawa Y, Tanaka M, Tanabe M, Suzuki T, Togawa T, Fukushige T, Kanekura T, Sakuraba H, Oishi K.  Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease.  PLoS One  2013  8(1):e55856  PubMed ID: 23383290
11715  Michihiko Sugimoto , Masayo Kondo, Michiko Hirose, Misao Suzuki, Kazuyuki Mekada, Takaya Abe, Hiroshi Kiyonari, Atsuo Ogura, Nobuo Takagi, Karen Artzt, Kuniya Abe  Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions  Cell Rep  2012  2(5):1363-74  PubMed ID: 23142660

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