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Cell No. : Cell Name
AES0136 : ZHBTc4  update : 2018/07/06
CommentMouse ES cell line derived from ZHTc6 and maintained by tetracycline(Tc)-regulatable Oct-3/4 transgene. Both of Oct-3/4 locus are disrupted by IRES-zeocin and IRES-BSD KO vectors. It should be cultured in the absence of tetraycline to maintain the undifferentiated state. See reference regarding more precise information.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Genet 2000;24:372) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Niwa, Hitoshi
Originator Niwa, Hitoshi
Year of deposit 2008
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Recombinant recombinant
Exogene Tetracycline-regulatable Oct3/4 transgene.
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 8
User's Publication 0


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Reference
4588  Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.  Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.  BMC Genomics  2008  9:269  PubMed ID: 18522731
4586  Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.  Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.  Nat Cell Biol  2007  9(6):625-35  PubMed ID: 17515932
4584  Nakatake Y, Fukui N, Iwamatsu Y, Masui S, Takahashi K, Yagi R, Yagi K, Miyazaki J, Matoba R, Ko MS, Niwa H.  Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells.  Mol Cell Biol  2006  26(20):7772-82  PubMed ID: 16954384
4585  Matoba R, Niwa H, Masui S, Ohtsuka S, Carter MG, Sharov AA, Ko MS.  Dissecting Oct3/4-regulated gene networks in embryonic stem cells by expression profiling.  PLoS One  2006  1:e26.  PubMed ID: 17183653
4582  Miyagi S, Saito T, Mizutani K, Masuyama N, Gotoh Y, Iwama A, Nakauchi H, Masui S, Niwa H, Nishimoto M, Muramatsu M, Okuda A.  The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells.  Mol Cell Biol  2004  24(10):4207-20  PubMed ID: 15121842
4579  Niwa H, Masui S, Chambers I, Smith AG, Miyazaki J.  Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells.  Mol Cell Biol  2002  22(5):1526-36  PubMed ID: 11839818
4578  Niwa H, Miyazaki J, Smith AG.  Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.  Nat Genet  2000  24(4):372-6  PubMed ID: 10742100
4580  Niwa H, Burdon T, Chambers I, Smith A.  Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3.  Genes Dev  1998  12(13):2048-60  PubMed ID: 9649508

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