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Cell No. : Cell Name
AES0135 : E14tg2a  update : 2018/07/06
CommentMouse embryonic stem (ES) cell line derived from 129/Ola.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nature 1987;326:292) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Niwa, Hitoshi
Originator Martin Hooper
Year of deposit 2008
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 40(47), 41(2), 42(1)
deposit info
lot info
Reference information Reference 2
User's Publication 9

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4604  Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H5.  Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells.  Development  2018  145(10):dev162404  PubMed ID: 29739838
4593  Hooper M, Hardy K, Handyside A, Hunter S, Monk M.  HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells.  Nature  1987  326(6110):292-5.  PubMed ID: 3821905

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User's Publication
6254  Maiko Yamamoto, Yoshiaki Suwa, Kohta Sugiyama, Naoki Okashita, Masanori Kawaguchi, Naoki Tani, Kazumi Matsubara, Akira Nakamura, Yoshiyuki Seki  The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency  J Cell Sci  2020   133(15):jcs240176  PubMed ID: 32661086
11578  Panina Y, Germond A, David BG, Watanabe TM.  Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay.  BMC Bioinformatics  2019    PubMed ID: 31146686
11703  Brit Gracy David, Hideaki Fujita, Kyota Yasuda, Kazuko Okamoto, Yulia Panina, Junya Ichinose, Osamu Sato, Masanobu Horie, Taro Ichimura, Yasushi Okada, Tomonobu M Watanabe  Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells  Stem Cell Research  2019    PubMed ID: 31715427
10741  Matsumiya M, Tomita T, Yoshioka-Kobayashi K, Isomura A, Kageyama R.  ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.  Development  2018  145:  PubMed ID: 29437832
10792  Kobayashi N, Kawasaki-Nishi S, Otsuka M, Hisano Y, Yamaguchi A, Nishi T.  MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells.  Sci Rep  2018  8:4969  PubMed ID: 29563527
11162  Okamoto K, Germond A, Fujita H, Furusawa C, Okada Y, Watanabe TM.  Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation.  Sci Rep  2018  8(1):11965  PubMed ID: 30097661
4081  Yulia Panina, Arno Germond, Brit G. David, Tomonobu M. Watanabe  Pairwise Efficiency: A new mathematical approach to qPCR data analysis increases the precision of the classical calibration curve assay  bioRxiv  2018   
10077  Ashida Y, Nakajima-Koyama M, Hirota A, Yamamoto T, Nishida E.  Activin A in combination with ERK1/2 MAPK pathway inhibition sustains propagation of mouse embryonic stem cells.  Genes Cells  2017  22:189-202  PubMed ID: 28097777
9720  Hidema S, Fukuda T, Date S, Tokitake Y, Matsui Y, Sasaki H, Nishimori K.  Transgenic expression of Telomerase reverse transcriptase (Tert) improves cell proliferation of primary cells and enhances reprogramming efficiency into the induced pluripotent stem cell.  Biosci. Biotechnol. Biochem.  2016  80:1925-33  PubMed ID: 27297181

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