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Cell No. : Cell Name
AES0135 : E14tg2a  update : 2022/05/27
CommentMouse embryonic stem (ES) cell line derived from 129/Ola.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nature 1987;326:292) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Niwa, Hitoshi
Originator Martin Hooper
Year of deposit 2008
Animal _mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_9108
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium GMEM + 10% FBS + 1mM Sodium Pyruvate + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 0.5-1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 40(47), 41(2), 42(1)
deposit info
lot info
Reference information Reference 2
User's Publication 15

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4604  Yamane M, Ohtsuka S, Matsuura K, Nakamura A, Niwa H5.  Overlapping functions of Krüppel-like factor family members: targeting multiple transcription factors to maintain the naïve pluripotency of mouse embryonic stem cells.  Development  2018  145(10):dev162404  PubMed ID: 29739838   DOI: 10.1242/dev.162404
4593  Hooper M, Hardy K, Handyside A, Hunter S, Monk M.  HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells.  Nature  1987  326(6110):292-5.  PubMed ID: 3821905   DOI: 10.1038/326292a0

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User's Publication
21396  Okamoto K, Fujita H, Okada Y, Shinkai S, Onami S, Abe K, Fujimoto K, Sasaki K, Shioi G, Watanabe TM.  Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.  EMBO J  2023  e112305  PubMed ID: 37609947   DOI: 10.15252/embj.2022112305
17678  Sanaki-Matsumiya M, Kageyama R.  Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo  Methods Mol Biol  2022  2525:321-332.  PubMed ID: 35836080   DOI: 10.1007/978-1-0716-2473-9_25
13386  Kaitsuka T, Tomizawa K.  Generation of Functional Insulin-Producing Cells from Mouse Embryonic Stem Cells Through Protein Transduction of Transcription Factors.  Methods Mol Biol  2021    PubMed ID: 33336272   DOI: 10.1007/978-1-0716-0943-9_7
20172  Ando Y, Okeyo KO, Sunaga J, Adachi T.  Edge-localized alteration in pluripotency state of mouse ES cells forming topography-confined layers on designed mesh substrates.  Stem Cell Res  2021  53:102352  PubMed ID: 33901814   DOI: 10.1016/j.scr.2021.102352
20195  Okamoto K, Watanabe TM, Horie M, Nishiyama M, Harada Y, Fujita H.  Pressure-induced changes on the morphology and gene expression in mammalian cells.  Biol Open  2021    PubMed ID: 34128043   DOI: 10.1242/bio.058544
20419  Kaneshiro J, Shioi G, Okamoto K, Onami S, Watanabe TM.  Improvement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy.  Opt Express  2021  29(15):24278-24288  PubMed ID: 34614676   DOI: 10.1364/OE.423783
6254  Maiko Yamamoto, Yoshiaki Suwa, Kohta Sugiyama, Naoki Okashita, Masanori Kawaguchi, Naoki Tani, Kazumi Matsubara, Akira Nakamura, Yoshiyuki Seki  The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency  J Cell Sci  2020   133(15):jcs240176  PubMed ID: 32661086   DOI: 10.1242/jcs.240176
11578  Panina Y, Germond A, David BG, Watanabe TM.  Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay.  BMC Bioinformatics  2019    PubMed ID: 31146686   DOI: 10.1186/s12859-019-2911-5
11703  Brit Gracy David, Hideaki Fujita, Kyota Yasuda, Kazuko Okamoto, Yulia Panina, Junya Ichinose, Osamu Sato, Masanobu Horie, Taro Ichimura, Yasushi Okada, Tomonobu M Watanabe  Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells  Stem Cell Research  2019    PubMed ID: 31715427   DOI: 10.1016/j.scr.2019.101614
12412  Ando Y, Okeyo KO, Adachi T.  Modulation of adhesion microenvironment using mesh substrates triggers self-organization and primordial germ cell-like differentiation in mouse ES cells  PL Bioeng  2019  3(1):016102  PubMed ID: 31069335   DOI: 10.1063/1.5072761
10741  Matsumiya M, Tomita T, Yoshioka-Kobayashi K, Isomura A, Kageyama R.  ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.  Development  2018  145:  PubMed ID: 29437832   DOI: 10.1242/dev.156836
10792  Kobayashi N, Kawasaki-Nishi S, Otsuka M, Hisano Y, Yamaguchi A, Nishi T.  MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells.  Sci Rep  2018  8:4969  PubMed ID: 29563527   DOI: 10.1038/s41598-018-23300-x
11162  Okamoto K, Germond A, Fujita H, Furusawa C, Okada Y, Watanabe TM.  Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation.  Sci Rep  2018  8(1):11965  PubMed ID: 30097661   DOI: 10.1038/s41598-018-30461-2
10077  Ashida Y, Nakajima-Koyama M, Hirota A, Yamamoto T, Nishida E.  Activin A in combination with ERK1/2 MAPK pathway inhibition sustains propagation of mouse embryonic stem cells.  Genes Cells  2017  22:189-202  PubMed ID: 28097777   DOI: 10.1111/gtc.12467
9720  Hidema S, Fukuda T, Date S, Tokitake Y, Matsui Y, Sasaki H, Nishimori K.  Transgenic expression of Telomerase reverse transcriptase (Tert) improves cell proliferation of primary cells and enhances reprogramming efficiency into the induced pluripotent stem cell.  Biosci. Biotechnol. Biochem.  2016  80:1925-33  PubMed ID: 27297181   DOI: 10.1080/09168451.2016.1191330

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