Cell No. : Cell Name
AES0134 : EGFP-MBD-nls
update : 2023/04/11
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| Comment | Mouse embryonic stem cells derived from 129/Ola male mouse. The methylated DNA binding domain (MBD) and the nuclear localization signal (nls) sequence coding for human methyl CpG-binding domain protein 1 (MBD1) were fused to the enhanced green fluorescent protein (EGFP) reporter gene, and ES cell lines carrying the construct ( EGFP-MBD-nls ) were established. Feeder-free cell line |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2007;12:447-460) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Abe, Kuniya
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| Originator |
Kobayakawa, Satoru
|
| Year of deposit |
2007
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| Another name |
RCB2708
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| Animal |
_mouse
< Mammals
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| Strain name |
129/Ola
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| Gender |
Male
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| Age at sampling |
embryo
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| Tissue |
embryonic stem cells
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| Classification |
ES
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| Recombinant |
recombinant
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| Exogene |
pCAGGS-EGFP-MBD-nls
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_U952
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose) + 10% FBS (HyClone) + 0.1mM NEAA + 55μM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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5-10 x 10 5 cells/60 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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free
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
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| User's Publication |
1
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