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Cell No. : Cell Name
AES0134 : EGFP-MBD-nls  update : 2018/07/06
CommentMouse embryonic stem cells derived from 129/Ola male mouse. The methylated DNA binding domain (MBD) and the nuclear localization signal (nls) sequence coding for human methyl CpG-binding domain protein 1 (MBD1) were fused to the enhanced green fluorescent protein (EGFP) reporter gene, and ES cell lines carrying the construct ( EGFP-MBD-nls ) were established. Feeder-free cell line
Comment from the depositor
Terms and conditions1) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.' 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2007;12:447-460) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Abe, Kuniya
Originator Kobayakawa, Satoru
Year of deposit 2007
Another name RCB2708
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene pCAGGS-EGFP-MBD-nls
Lifespan infinite
Morphology ES-like
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Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS (HyClone) + 0.1mM NEAA + 55μM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-10 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
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Reference information Reference 1
User's Publication 0

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4592  Kobayakawa S, Miike K, Nakao M, Abe K.  Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells.  Genes Cells  2007  12(4):447-60  PubMed ID: 17397393

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