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Cell No. : Cell Name
AES0134 : EGFP-MBD-nls  update : 2022/05/27
CommentMouse embryonic stem cells derived from 129/Ola male mouse. The methylated DNA binding domain (MBD) and the nuclear localization signal (nls) sequence coding for human methyl CpG-binding domain protein 1 (MBD1) were fused to the enhanced green fluorescent protein (EGFP) reporter gene, and ES cell lines carrying the construct ( EGFP-MBD-nls ) were established. Feeder-free cell line
Comment from the depositor
Terms and conditions1) The RECIPIENT should read Announcement of GFP bioresources' 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2007;12:447-460) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Abe, Kuniya
Originator Kobayakawa, Satoru
Year of deposit 2007
Another name RCB2708
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene pCAGGS-EGFP-MBD-nls
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_U952
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS (HyClone) + 0.1mM NEAA + 55μM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-10 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
4592  Kobayakawa S, Miike K, Nakao M, Abe K.  Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells.  Genes Cells  2007  12(4):447-60  PubMed ID: 17397393   DOI: 10.1111/j.1365-2443.2007.01063.x

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User's Publication
20128  Wang W, Ito T, Otsuka S, Nansai H, Abe K, Nakao Y, Ohgane J, Yoneda M, Sone H.  Epigenetic effects of insecticides on early differentiation of mouse embryonic stem cells.  Toxicol In Vitro  2021  105174  PubMed ID: 33865946   DOI: 10.1016/j.tiv.2021.105174



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