Search
CELL BANK Website

Back Back
Cell No. : Cell Name
AES0134 : EGFP-MBD-nls  update : 2018/07/06
CommentMouse embryonic stem cells derived from 129/Ola male mouse. The methylated DNA binding domain (MBD) and the nuclear localization signal (nls) sequence coding for human methyl CpG-binding domain protein 1 (MBD1) were fused to the enhanced green fluorescent protein (EGFP) reporter gene, and ES cell lines carrying the construct ( EGFP-MBD-nls ) were established. Feeder-free cell line
Comment from the depositor
Terms and conditions1) The RECIPIENT should read the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.' 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Genes Cells 2007;12:447-460) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Abe, Kuniya
Originator Kobayakawa, Satoru
Year of deposit 2007
Another name RCB2708
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Recombinant recombinant
Exogene pCAGGS-EGFP-MBD-nls
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS (HyClone) + 0.1mM NEAA + 55μM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-10 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


To topTop
Reference
4592  Kobayakawa S, Miike K, Nakao M, Abe K.  Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells.  Genes Cells  2007  12(4):447-60  PubMed ID: 17397393

To topTop
User's Publication



Back Back Return Top Page