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Cell No. : Cell Name
AES0131 : BXM7  update : 2018/07/06
CommentMouse embryonic stem cell line derived from F1 mouse (C57BL/6 x MSM).
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Reprod Dev 2007;74:1081-1036) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko
Year of deposit 2007
Another name RCB2705
Animal mouse < Mammals
Strain name C57BL/6xMSM
Gender Unknown
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10^(5) cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3604  Shinmen A, Honda A, Ohkawa M, Hirose M, Ogonuki N, Yuzuriha M, Miki H, Mochida K, Inoue K, Abe K, Ito M, Ogura A.  Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection.  Mol Reprod Dev  2007  74(9):1081-8  PubMed ID: 17290420

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