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Cell No. : Cell Name
AES0129 : HSNT2  update : 2018/07/06
CommentMouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus (Hematopoietic stem cell) was derived from F1 (C57BL/6 x 129) male mouse.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Curr Biol 2005;15:1114-1118, J Cell Sci 2006;119:1985-1989) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ogura, Atsuo
Originator Hirose, Michiko
Year of deposit 2007
Another name RCB2703
Animal mouse < Mammals
Strain name (C57BL/6 x 129)F1
Gender Male
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
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Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% trypsin + 0.02% EDTA
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 2 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells MEF
Feeder treatment X-rays 5000R (or MMC)
Feeder seed cells 2.5-5.0 x 10^(5) cells/60 mm dish
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
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Reference information Reference 2
User's Publication 0

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3603  Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim JM, Aoki F, Miyoshi H, Ogura A.  Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer.  J Cell Sci  2006  119(Pt 10):1985-91  PubMed ID: 16621892
3602  Inoue K, Wakao H, Ogonuki N, Miki H, Seino K, Nambu-Wakao R, Noda S, Miyoshi H, Koseki H, Taniguchi M, Ogura A.  Generation of cloned mice by direct nuclear transfer from natural killer T cells.  Curr Biol  2005  15(12):1114-8  PubMed ID: 15964276

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