Cell No. : Cell Name
AES0128 : HSNT1
update : 2023/04/11
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| Comment | Mouse embryonic stem (ES) cell line established by nuclear transfer technique. Nucleus (Hematopoietic stem cell) was derived from F1 (C57BL/6 x 129) male mouse. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Curr Biol 2005;15:1114-1118, J Cell Sci 2006;119:1985-1989) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Ogura, Atsuo
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| Originator |
Hirose, Michiko
|
| Year of deposit |
2007
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| Another name |
RCB2702
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| Animal |
_mouse
< Mammals
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| Strain name |
(C57BL/6 x 129)F1
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| Gender |
Male
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| Tissue |
embryonic stem cells
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| Classification |
ES
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|
Lifespan |
infinite
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| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_K096
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (high glucose) + 15% KSR + 0.1mM NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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2 x 10 5 cells/60 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Feeder cells |
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MEF
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| Feeder treatment |
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X-rays 5000R (or MMC)
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| Feeder seed cells |
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2.5-5.0 x 10 5 cells/60 mm dish
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| Freeze medium |
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Medium : 2 x FM1 = 1 : 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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| Reference |
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3603
Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim JM, Aoki F, Miyoshi H, Ogura A.
Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer.
J Cell Sci
2006
119(Pt 10):1985-91
PubMed ID: 16621892
DOI: 10.1242/jcs.02913
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3602
Inoue K, Wakao H, Ogonuki N, Miki H, Seino K, Nambu-Wakao R, Noda S, Miyoshi H, Koseki H, Taniguchi M, Ogura A.
Generation of cloned mice by direct nuclear transfer from natural killer T cells.
Curr Biol
2005
15(12):1114-8
PubMed ID: 15964276
DOI: 10.1016/j.cub.2005.05.021
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