Cell No. : Cell Name
AES0125 : 2TS22C
update : 2022/12/21
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| Comment | Mouse embryonic stem cells derived from 129/Ola male mouse. Feeder-free cell line |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Cell Biol 2007;9:625-635) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0032.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Masui, Shinji
|
| Originator |
Masui, Shinji
|
| Year of deposit |
2007
|
| Another name |
RCB2690
|
| Animal |
_mouse
< Mammals
|
| Strain name |
129/Ola
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonic stem cells
|
| Classification |
ES
|
|
Lifespan |
infinite
|
| Morphology |
ES-like
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| Cellosaurus(Expasy) |
CVCL_E266
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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Stem Medium (or Culti Cell) + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
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| Coating dish |
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0.1% gelatin coated dish
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Culture information
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Passage cell No |
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5-10 x 10 5 cells/100 mm dish
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| SC frequency |
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Subculture : once/2-3 days
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| Temperature |
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37
℃
|
| CO2 concentration |
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5
%
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| Feeder cells |
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free
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| Freeze medium |
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Medium : 2 x FM1 = 1 : 1
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
2
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| Reference |
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4588
Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.
Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.
BMC Genomics
2008
9:269
PubMed ID: 18522731
DOI: 10.1186/1471-2164-9-269
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4586
Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.
Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.
Nat Cell Biol
2007
9(6):625-35
PubMed ID: 17515932
DOI: 10.1038/ncb1589
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| User's Publication |
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13422
Friman, Torgil Elias
The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells
Lausanne, EPFL
2019
DOI: 10.5075/epfl-thesis-9790
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9968
Ochiai W, Hirose A, Kawamura T, Komachi K, Yamamoto Y, Kitaoka S, Hatogai J, Kusunoki Y, Kon R, Ikarashi N, Sugiyama K.
Role of the Drug-Metabolizing Enzyme CYP during Mouse Liver Development.
Biol. Pharm. Bull.
2016
39:2015-2021
PubMed ID: 27904043
DOI: 10.1248/bpb.b16-00479
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