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Cell No. : Cell Name
AES0125 : 2TS22C  update : 2022/05/27
CommentMouse embryonic stem cells derived from 129/Ola male mouse. Feeder-free cell line
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Nat Cell Biol 2007;9:625-635) designated by the DEPOSITOR is required.
Order Form Order Form(C-0032.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Masui, Shinji
Originator Masui, Shinji
Year of deposit 2007
Another name RCB2690
Animal mouse < Mammals
Strain name 129/Ola
Gender Male
Age at sampling embryo
Tissue embryonic stem cells
Classification ES
Lifespan infinite
Morphology ES-like
Cellosaurus(Expasy) CVCL_E266
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Stem Medium (or Culti Cell) + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse LIF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Coating dish 0.1% gelatin coated dish
Culture information Passage cell No 5-10 x 10^(5) cells/100 mm dish
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Feeder cells free
Freeze medium Medium : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 2

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4588  Sharov AA, Masui S, Sharova LV, Piao Y, Aiba K, Matoba R, Xin L, Niwa H, Ko MS.  Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data.  BMC Genomics  2008  9:269  PubMed ID: 18522731   DOI: 10.1186/1471-2164-9-269
4586  Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K, Okochi H, Okuda A, Matoba R, Sharov AA, Ko MS, Niwa H.  Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.  Nat Cell Biol  2007  9(6):625-35  PubMed ID: 17515932   DOI: 10.1038/ncb1589

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User's Publication
13422  Friman, Torgil Elias  The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells  Lausanne, EPFL  2019      DOI: 10.5075/epfl-thesis-9790
9968  Ochiai W, Hirose A, Kawamura T, Komachi K, Yamamoto Y, Kitaoka S, Hatogai J, Kusunoki Y, Kon R, Ikarashi N, Sugiyama K.  Role of the Drug-Metabolizing Enzyme CYP during Mouse Liver Development.  Biol. Pharm. Bull.  2016  39:2015-2021  PubMed ID: 27904043   DOI: 10.1248/bpb.b16-00479

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